卵巢癌SKOV3细胞培养液对脐血树突细胞前体细胞亚群的影响  

Influence of SKOV3 culture medium on subset shift of dendritic cell presursors from cord blood in vitro

作　　者：余艳[1] 陈丽莉[1] 叶枫[1] 吕卫国[1] 谢幸[1] 

机构地区：[1]浙江大学医学院附属妇产科医院浙江省女性生殖与健康重点实验室,杭州310006

出　　处：《现代妇产科进展》2008年第5期350-353,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:30471811)

摘　　要：目的:研究卵巢癌细胞培养上清能否诱导树突细胞前体细胞亚群比例发生变化。方法:以脐血来源的CD34+干细胞通过细胞因子FLT3-L(100ng/ml)、SCF(50ng/ml)的诱导在体外扩增分化为DC前体细胞(pre-DC)。培养并收集卵巢癌细胞株SKOV3上清,经透析法浓缩。将上清培养的pre-DC,经不同浓度上清(0%、25%、50%、75%、100%、150%)作用后,用流式细胞仪(flowcytometry,FCM)检测pre-DC表面抗原CD123、CD11c、HLA-DR、CD80、CD86的表达。结果:上清促进CD11c和HLA-DR抗原的表达,不促进CD123、CD80、CD86抗原的表达。结论:卵巢癌细胞株上清影响两类DC前体细胞亚群的比例变化,可能通过此途径参与形成腹腔免疫缺陷。Objective：To investigate whether the supernatant from cultured ovarian carcinomas cell line SKOV3 could influence subset shift of dendritic cell presursors （pre-DC）. Methods：CD34 ^＋ hematopoietic stem cells were induced by cytokines FLT3-L（100ng/ml） and SCF （50ng/ml） in vitro. The supernate of ovarian carcinoma cell lines （ SKOV3 ） was cultivated and collected and the supernate was concentrated by dialysis. The expression of pre-DC was induced by the supernate with different concentrations （0% ,25% ,50% ,75%, 100%, 150% ）. The expression of pre-DC was detected by flow cytometry. Results：The supernate of ovarian carcinoma cell lines could promote the expression of CD11 c, HLA-DR. The supernate of ovarian carcinoma cell lines could not promote the expression of CD123, CD80, CD86. Conclusion ：The supernate of SKOV3 cell lines can regulate the subset ratio of dendritic cell precursors in vitro, which may be involved intraperitoneal immunodeficiency.

关 键 词：卵巢肿瘤 树突细胞前体细胞 亚群比例 体外 

分 类 号：R737.31[医药卫生—肿瘤]