NuMA和γ-tubulin在核移植克隆胚胎中的动态变化和作用  

Dynamic Changes and Functions of NuMA andγ-tubulin in Nuclear Transfer Cloned Embryos

作　　者：张庆华[1] 关娜[1] 徐燕宁[1] 钟淑琦[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组织胚胎学教研室,黑龙江哈尔滨150081

出　　处：《解剖科学进展》2008年第2期216-220,共5页Progress of Anatomical Sciences

摘　　要：核有丝分裂器蛋白(NuMA)和γ-微管蛋白(γ-tubulin)在卵母细胞成熟、受精和核移植胚胎早期发育过程中起重要作用。供体细胞γ-tubulin和NuMA参与了核移植克隆胚有丝分裂纺锤体的组装,NuMA主要参与了纺锤体极的装配和维持,而供体中心体调节核移植胚最初纺锤体的形成。核移植重构胚中心体结构异常经常伴随染色体异常排列、纺锤体结构异常,而中心体分布异常经常伴随异常卵裂。Nuclear mitotic apparatus protein （NuMA） and γ-tubulin play important roles in the oocyte maturation, fertilization, and preimplantation development of nuclear transfer cloned embryos. In somatic cell nuclear transferred embryos, NuMA and γ-Tubulin derived from the donor cell promote the mitotic spindle assembly. NuMA involves in the formation and maintenance of spindle pole. However, donor centrosome regulates the formation of the initial spindle in nuclear transferred embryos. Aberrant construction of centrosomes is associated with the formation of abnormal spindles and chromosome misaliguments while abnormal distribution of centrosomes coincides with abnormal cleavage.

关 键 词：胚胎早期 TUBULIN 核移植 克隆胚 动态变化 核有丝分裂器蛋白 卵母细胞成熟 供体细胞 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学] R339.14[医药卫生—基础医学]