雌性藏猪不同生长发育阶段肌肉生长和脂肪沉积相关基因的表达模式  

Expression patterns of genes associated with muscle growth and adipose deposition during growth and development of female Tibetan pigs

作　　者：李明洲[1] 李学伟[1] 帅素容[1] 朱砺[1] 刘海峰[1] 王金勇 蒋忠荣 滕晓坤[4] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]重庆市畜牧科学研究院,重庆荣昌402460 [3]四川省甘孜藏族自治州畜牧业科学研究所,四川康定626000 [4]生物芯片上海国家工程研究中心,上海201203

出　　处：《动物学报》2008年第3期442-452,共11页ACTA ZOOLOGICA SINICA

基　　金：教育部长江学者和创新团队发展计划(No.IRT0555-6);教育部高等学校博士学科点专项基金(No.20060626003);国家科技支撑计划(No.2007BAD51B03);四川省教育厅青年基金(No.2006B004)资助项目~~

摘　　要：本研究利用包含140个与猪肌肉生长和脂肪沉积密切相关基因的Oligo功能分类基因芯片检测了藏猪在2、4、6和8月龄间背最长肌中这些基因的表达量变化,并在2月龄时与脂肪型的太湖猪和瘦肉型的长白猪进行比较。ANOVA分析结果表明:2-8月龄间藏猪分别有10和7个基因的表达差异达极显著(P<0.01)和显著水平(P<0.05);2月龄时藏猪体重极显著低于长白猪(P<0.01)和显著低于太湖猪(P<0.05),而藏猪肌纤维面积却为最大,但品种间差异未达显著水平(P>0.05);2月龄时3个品种间分别有15和13个基因的表达差异达极显著(P<0.01)和显著水平(P<0.05)。STEM聚类分析结果表明:直线下降和上升是藏猪在2-8月龄间最具代表性的基因表达模式(P<0.01)。另外,5个差异表达基因的荧光定量RT-PCR验证结果与基因芯片结果的Person相关系数平均高达0.856±0.109。提示:藏猪在2-8月龄间骨骼肌生长发育强度较肌内脂肪合成沉积占优势,2月龄时藏猪脂肪酸合成相关基因的表达水平较其他两品种低,而脂肪酸β氧化和肌纤维生长相关基因的表达水平较高,与其在高原独特的自然生态环境和全放牧散养的饲喂方式下长期形成的品种特性相符。Changes in expression of 140 genes associated with growth and adipose deposition in the logissimus dorsi muscle were studied at various growth stages, in Tibetan, Taihu and Landrace pigs, using a pathway-focused oligo microarray. Analysis of variance （ANOVA） revealed statistically significant differences in the expression of 17 genes in Tibetan pigs at 4 stages （2, 4, 6 and 8 months of age）. Significant differerces were also identified in the expression of 28 genes, at 2 months of age, in all 3 pig breeds. Tibetan pigs had significantly lower body weights than Taihu and Landrace pigs, at two months, and a larger myofiber cross-sectional area （CSA）. Cluster analysis revealed a significant （P 〈 0.01 ） level of expression of 2 genes in Tibetan pigs, and strong down-regulation, or up-regulation of genes between 2 - 8 months. Quantitative, real time RT-PCR was used to verify the microarray data for five modulated genes, a good correlation between the two measures was observed for Tibetan pigs at different growth stages （R = 0.856±0.109）. These results indicate that, in Tibetan pigs aged 2 - 8 months, growth intensity for muscle is higher than for intramuscular fat. The genes that exhibited down-regulation were mainly those controlling fatty acid biosynthesis. Genes that were up-regulated were concerned mainly with fatty acid β-oxidation and myofibre growth; more so than in Tibetan pigs than in the other breeds at 2 months of age. These results are consistent with the unique genetic characteristics of Tibetan pigs, which are adapted to unusual ecological conditions in the high plateau areas.

关 键 词：藏猪 肌肉 肌内脂肪 基因芯片 

分 类 号：S828[农业科学—畜牧学]