低氧习服对模拟高原低氧大鼠肺组织的影响  

Effect of hypoxic preconditioning on rat lungs exposed to simulated high altitude hypoxia

作　　者：李福祥[1] 夏前明[1] 李鸿雁[1] 全燕[1] 肖贞良[2] 张彦[1] 连亨宁 胡绳[1] 

机构地区：[1]解放军成都军区总医院呼吸内科,四川成都610083 [2]解放军成都军区总医院重症加强治疗病房,四川成都610083

出　　处：《中国呼吸与危重监护杂志》2008年第3期199-204,I0002,共7页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：中国人民解放军全军医药卫生科研基金(编号:01MB038)

摘　　要：目的观察低氧习服对模拟高原肺水肿大鼠肺组织结构及低氧诱导因子1α(HIF-1α)表达的影响。方法56只Wistar大鼠随机分为7组:常氧对照组(N组)、急性低氧对照组(H0组)、急性低氧组(H1组)、3000m低氧习服组(C3.0组)、3000m低氧习服+急性低氧组(C3.1组)、5000m低氧习服组(C5.0组)、5000m低氧习服+急性低氧组(C5.1组),每组8只。习服完成后,将大鼠置于模拟海拔6000m急性低氧24h。采用Westernblot和RT-PCR方法检测动物习服完成后肺组织HIF-1α蛋白和mRNA的表达情况,光镜和电镜下观察大鼠急性低氧后肺结构变化。结果H1组肺组织显微和超微结构出现明显间质性肺水肿,低氧习服后间质性肺水肿明显减轻。Westernblot方法未检测到大鼠肺组织表达HIF-1α,但HIF-1αmRNA在N组有一定量表达,C3.0组和C5.0组HIF-1αmRNA表达均明显高于N组(P均<0.01)。结论低氧习服可以减轻低氧大鼠肺间质水肿程度,提高肺组织HIF-1αmRNA表达。HIF-1可能介导参与了大鼠肺低氧习服过程。Objective To investigate the influence of hypoxic preconditioning on pulmonary structure of rats exposed to simulated high altitude hypoxia and explore the role of hypoxia inducible factor-1α（HIF-1α）.Methods Fifty-six Wistar rats were randomly divided into 7 groups（n=8 in each group）,ie,a normal control group（N group）,an acute hypoxic control group（H0 group）,an acute hypoxic group（H1 group）,a 3000 m hypoxic preconditioning group（C3.0 group）,a 3000 m hypoxic preconditioning ＋ acute hypoxic group（C3.1 group）,a 5000 m hypoxic preconditioning group（C5.0 group）,and a 5000 m hypoxic preconditioning ＋ acute hypoxic group（C5.1 group）.After treated with hypoxic preconditioning,the animals were exposed to simulated altitude of 6000 m for 24 hours.Then the protein and mRNA expression of HIF-1α in lung of N,H0,C3.0 and C5.0 groups were assessed by Western blot and RT-PCR,respectively.The lung structure in N,H1,C3.1 and C5.1 groups was observed by light microscope and electron microscope.Results Pulmonary interstitial edema was apparently observed in H1 group,while significantly relieved in two hypoxic preconditioning groups.HIF-1α protein was not detected in rat lungs by Western blot analysis.Compared to N group,the levels of HIF-1α mRNA significantly increased in C3.0 group and C5.0 group（both P〈0.01）.Conclusions Hypoxic preconditioning can relieve hypoxic pulmonary interstitial edema and increase HIF-1α mRNA expression in rat lungs.HIF-1 may be involved in the process of hypoxic preconditioning in rat lungs.

关 键 词：高原肺水肿 低氧习服 低氧诱导因子1 

分 类 号：R363[医药卫生—病理学]