Differential gene expression profiling of human epidermal growth factor receptor 2- overexpressing mammary tumor  

作　　者：Yan Wang Haining Peng Yingli Zhong Daiqiang Li Mi Tang Xiaofeng Ding Jian Zhang 

机构地区：[1]Key Laboratory of Protein Chemistry and Developmental Biology of the Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China [2]Department of Pathology, the Second Xiangya Hospital, Central [3]Model Organism Division, E-Institutes of Shanghai Universities, South University, Changsha 410011, China Shanghai Second Medical University, Shanghai 200025, China

出　　处：《Acta Biochimica et Biophysica Sinica》2008年第5期397-405,共9页生物化学与生物物理学报（英文版）

基　　金：This work was supported in part by the grants from the National Natural Science Foundation of China （20335020 and 90608006）, the Program for Changjiang Scholars and Innovative Research Team in University （No. IRT0445）, and the Science and Technology Department of Hunan Province （No. 2006JT2008） We gratefully acknowledge the Department of Pathology, the Second Xiangya Hospital, Central South University （Changsha, China） for their role in obtaining breast cancer tissue samples.

摘　　要：Human epidermal growth factor receptor 2 （HER2） is highly expressed in approximately 30% of breast cancer patients, and substantial evidence supports the relationship between HER2 overexpression and poor overall survival. However, the biological function of HER2 signaltransduction pathways is not entirely clear. To investigate gene activation within the pathways, we screened differentially expressed genes in HER2-positive mouse mammary tumor using two-directional suppression subtractive hybridization combined with reverse dot-blotting analysis. Forty genes and expressed sequence tags related to transduction, cell proliferation/growth/ apoptosis and secreted/extracellular matrix proteins were differentially expressed in HER2-positive mammary tumor tissue. Among these, 19 were already reported to be differentially expressed in mammary tumor, 11 were first identified to be differentially expressed in mammary tumor in this study but were already reported in other tumors, and 10 correlated with other cancers. These genes can facilitate the understanding of the role of HER2 signaling in breast cancer.Human epidermal growth factor receptor 2 （HER2） is highly expressed in approximately 30% of breast cancer patients, and substantial evidence supports the relationship between HER2 overexpression and poor overall survival. However, the biological function of HER2 signaltransduction pathways is not entirely clear. To investigate gene activation within the pathways, we screened differentially expressed genes in HER2-positive mouse mammary tumor using two-directional suppression subtractive hybridization combined with reverse dot-blotting analysis. Forty genes and expressed sequence tags related to transduction, cell proliferation/growth/ apoptosis and secreted/extracellular matrix proteins were differentially expressed in HER2-positive mammary tumor tissue. Among these, 19 were already reported to be differentially expressed in mammary tumor, 11 were first identified to be differentially expressed in mammary tumor in this study but were already reported in other tumors, and 10 correlated with other cancers. These genes can facilitate the understanding of the role of HER2 signaling in breast cancer.

关 键 词：mammary tumor HER2 suppression subtractive hybridization 

分 类 号：R737.9[医药卫生—肿瘤]