Tretinoin or retinol enhancement of lymphokine-activated killer cell proliferation and cytotoxicity against human bladder cancer cells in vitro  

作　　者：王志平[1,2] 郑荣梁[1,2] 陈一戎[1,2] 刘国栋 

机构地区：[1]兰州大学生物系 [2]兰州医学院第二附属医院泌尿外科研究所

出　　处：《中国药理学报》1997年第6期519-521,共3页Acta Pharmacologica Sinica

摘　　要：目的：研究维Ａ酸（Ｔｒｅ）或维生素Ａ（Ｒｅｔ）对膀胱肿瘤患者淋巴因子激活的杀伤细胞（ＬＡＫ细胞）的增殖和对膀胱肿瘤细胞的细胞毒作用．方法：分别用细胞计数和ＭＴＴ法测定ＬＡＫ细胞的增殖和细胞毒作用．结果：Ｔｒｅ或Ｒｅｔ１０－１００ｎｍｏｌ·Ｌ－１加强由白细胞介素２（ＩＬ２）诱导的ＬＡＫ细胞的增殖和对膀胱肿瘤细胞的杀伤作用．结论：Ｔｒｅ或Ｒｅｔ增强膀胱肿瘤患者ＬＡＫ细胞增殖及对膀胱肿瘤的细胞毒作用．IM: To study the effect of tretinoin (Tre) or retinol (Ret) on the proliferation of lymphokine activated killer (LAK) cells in patients with transitional cell cancer of bladder and their cytolysis to bladder tumor cells. METHODS: LAK cell proliferation was assayed in the presence of either Tre or Ret by cell counting. Human transitional bladder cancer cell lines BIU 87, EJ, or bladder tumor cells (BTC) from patients with bladder cancer were used as target cells and cytotoxicity of LAK cells was determined by 3 (4,5 Dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. RESULTS: The proliferation of LAK cells induced by interleukin 2 (IL 2) was stimulated by Tre or Ret (10-100 nmol·L -1 ). The cytotoxicity of LAK cells against BIU 87, EJ cells, or BTC was enhanced by pretreatment of LAK cells with Tre or Ret 10-100 nmol·L -1 . CONCLUSION: Tre or Ret enhances the proliferation and cytotoxicity of LAK cells from patients with bladder cancer. Retinoids are potential in adoptive immunotherapy of bladder cancer.

关 键 词：维A酸 维生素A 淋巴因子激活 免疫细胞 毒性 

分 类 号：R737.140.5[医药卫生—肿瘤]