颈交感神经干离断对大鼠局灶性脑缺血再灌注损伤的影响  

Effects of transection of cervical sympathetic trunk on focal cerebral ischemia/reperfusion injury in rats

作　　者：熊良志[1] 史永霞[1] 王清秀[2] 肖峰[1] 

机构地区：[1]郧阳医学院太和医院麻醉科,湖北省十堰市442000 [2]同济大学东方医院麻醉科,上海市200120

出　　处：《实用医学杂志》2008年第10期1683-1686,共4页The Journal of Practical Medicine

基　　金：湖北省教育厅优秀中青年人才项目(编号:2002B03001)

摘　　要：目的:采用颈交感干离断(TCST)模拟星状神经节阻滞,观察其对大鼠局灶性脑缺血再灌注损伤的影响。方法:将实验大鼠随机分成实验组、对照组和假手术组。采用血管内线栓法行大脑中动脉栓塞制作局灶性脑缺血再灌注模型,缺血2h再灌注24h。观察TCST对局灶性脑缺血大鼠神经行为学评分、脑梗死容积、缺血侧脑组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量及一氧化氮(NO)水平的影响。结果:与对照组相比,实验组大鼠神经行为学评分和脑梗死容积明显降低(P<0.05),脑组织SOD活力增加,而MDA含量和NO水平显著降低(P<0.05)。结论:TCST对局灶性脑缺血再灌注损伤具有保护作用,其作用可能与其提高SOD活力、降低MDA含量和NO水平有关。Objective To investigate the effects of transection of cervical sympathetic trunk （TCST） on focal cerebral ischemia/reperfusion injury in rat by simulating stellate ganglion block. Methods Rats were randomly assigned to experimental group, control group, or sham-operation group. The rat model of focal cerebral ischemia/reperfusion was established by middle cerebral artery occlusion via an intravascular thread insertion, with 2-hour ischemia followed by 24 hours of reperfusion. Observed the effects of TCST on neurological deficit scores, cerebral infarct size, superoxide dismutase （SOD） activity, malondialdehyde （MDA） content, and nitric oxide （NO） level in the ischemic tissue of the brain in rats were observed. Results As compared with those in the control group, in the experimental group, neurological deficit scores and cerebral infarct size were significantly reduced, as were MDA content and NO level; while SOD activity was increased （P 〈 0.05 for all comparisons）. Conclusion TCST has a neuroproteetive effect on focal cerebral ischemia/reperfusion injury, which may be associated with its action to increase SOD activity and decrease both MDA content and NO level.

关 键 词：再灌注损伤 脑 肾上腺素能纤维 自由基一氧化氮 

分 类 号：R743[医药卫生—神经病学与精神病学]