转化生长因子β_1在下颌压缩成骨过程中的表达研究  

Expression of transforming growth factor-beta 1 in mandibular condensability and length contraction

作　　者：杨宓[1] 邓锋[1] 李勇[2] 张定铭[1] 汪克建[3] 

机构地区：[1]重庆医科大学附属口腔医院口腔正畸科,400015 [2]重庆医科大学附属口腔医院口腔颌面外科,400015 [3]重庆医科大学基础医学院病理教研室,400016

出　　处：《重庆医学》2008年第11期1189-1190,1194,共3页Chongqing medicine

基　　金：重庆市自然科学基金资助项目(渝科发计字[2004]54号)

摘　　要：目的研究转化生长因子β1(transforming growth factor β1,TGF-β1)在下颌压缩成骨过程中的表达及意义。方法(1)山羊8只,单侧实验,口外进路,下颌角区保留骨松质连续性的颊舌侧骨皮质切开术,采用特制压缩器械,每3天加力1次,每次压缩0.5mm;(2)分别在压缩结束后0、7、14、28d处死2只动物,切取压缩区骨组织脱钙处理、切片,免疫组化观察TGF-β1在下颌压缩成骨过程中的表达强度。结果(1)颌骨长度被成功缩短;(2)TGF-β1在下颌压缩成骨过程中呈与时间相关的波形表达,主要定位于成纤维细胞、成软骨细胞和成骨细胞的胞浆中,其中以压缩结束第7天的表达最强,第14天逐渐下降,28d时仅有微弱表达。结论压缩成骨的过程中,机械压缩力刺激,可使局部压缩区TGF-β1持续较高表达;持续较高表达的TGF-β1还可能参与促进压缩区新骨的形成。Objective To investigate the expression of TGF-β1 in the contracted area after the the mandibular length is shortened. Methods （1）Eight goats were used in the study. Corti-cotomy at right mandibular angles were performed via extral-oral accession. Special devices were applied to shorten the mandibles by 0.5 mm per three days respectively. （2）Two goats were killed respectively on 0,7,14,28d after the mandibular length was shortened. The contracted areas were harvested and processed for immunohistochemical detection of TGF-β1 existed. Results （1）Mandibular angles of the 8 goats were shorted successfully. （2）The higher expression of TGF-β1 mainly located in fibroblasts,chondroblasts and osteoblasts. The level of TGF-β1 began to climb higher and higher since the end of contraction, reached to the peak after 7d of fixed time and declined gradually from 7d to 14d,and after 28d it became weak. Conclusion The mechanical press might activate a high level expression of TGF-β1 in the contracted zone during contraction osteogenesis,and TGF-β1 appears to play an important role in bone regeneration in the contracted zone.

关 键 词：颌骨 骨压缩 压缩成骨 转化生长因子Β1 

分 类 号：R782[医药卫生—口腔医学]