乳腺癌中NDRG-1基因甲基化状态研究  

The methylation status of CpG island in the promoter region of NDRG-1 gene in breast cancer

作　　者：王立玮[1] 周东蕊[2] 房汝敬[2] 张仁敏[2] 陆祖宏[2] 何杰[1] 

机构地区：[1]东南大学临床医学院东南大学附属中大医院病理科,江苏南京210009 [2]东南大学分子电子学国家重点实验室,江苏南京210096

出　　处：《现代医学》2008年第2期79-83,共5页Modern Medical Journal

摘　　要：目的研究NDRG1基因启动子区甲基化状态,探讨NDRG-1基因甲基化在乳腺癌发生中的作用。方法采用双色荧光杂交微阵列基因芯片技术对33对乳腺癌标本和癌旁组织标本NDRG-1基因启动子区甲基化状态进行研究。结果肿瘤标本和癌旁组织标本中NDRG-1基因启动子区CpG位点均有不同程度的甲基化,肿瘤组织中NDRG-1基因启动子区CpG位点甲基化率明显高于相应的癌旁组织(t=14.12,P<0.05)。结论NDRG-1基因启动子区过甲基化可能在乳腺癌的发生过程中起重要作用。Objective To explore the correlations of methylation status in the promoter region of NDRG1 gene to carcinogenesis of breast cancer. Methods Microarray-based methylation analysis was used to detect the methylation status in the promoter region of NDRG1 gene in 33 pairs of breast cancer and corresponding normal tissues. Results The metbylation level of CpG island in the promoter region of NDRG1 gene in breast cancer was higher than that in tissues adjacent （t = 14.12,P 〈 0.05 ）. Conclusion Hypermethylation of CpG island in the promoter region of NDRG-1 gene possibly plays important role in carcinogenesis of breast cancer.

关 键 词：乳腺癌 甲基化 NDRG-1基因 

分 类 号：R735[医药卫生—肿瘤]