糖基化终产物对视网膜Müller细胞表达碱性成纤维细胞生长因子的影响  

Effect of advanced glycosylation end products on expression of basic fibroblast growth factor in cultured rabbit retinal Müller cells

作　　者：艾静[1] 刘瑶[1] 栾洁[1] 陈平圣[2] 

机构地区：[1]东南大学附属中大医院眼科,江苏南京210009 [2]东南大学基础医学院病理学教研室,江苏南京210009

出　　处：《现代医学》2008年第2期107-110,共4页Modern Medical Journal

摘　　要：目的探讨糖基化终产物(AGEs)对体外培养兔视网膜Müller细胞表达碱性成纤维细胞生长因子(bFGF)的影响。方法体外分离并培养兔视网膜Müller细胞。37℃条件下制备牛血清白蛋白AGEs(AGEs-BSA)及其对照物。应用AGEs-BSA及其对照物作用于Müller细胞,采用免疫细胞化学(ICC)方法半定量检测AGEs对视网膜Müller细胞表达bFGF的影响。结果与对照组相比,AGEs作用下体外培养的兔视网膜Müller细胞bFGF的表达增高(P<0.05或P<0.01),且具有一定的时间和浓度依赖性。结论AGEs可以上调Müller细胞表达bFGF,推测AGEs可能通过增加bFGF的表达从而加速糖尿病视网膜病变(DR)新生血管的形成。Objective To investigate the effect of advanced glycosylation end products（AGEs）on expression of basic fibroblast growth factor （bFGF） in rabbit retinal Müller cells in vitro. Methods Rabbit retinal Müller cells were cultured according to the descriptions in literatures. AGEs-BSA and its control were made in 37℃ in vitro. Müller cells were divided into AGEs-BSA group, AGEs-BSA control group and blank control group. AGEs-BSA group and AGEs-BSA control group were respectively treated with 5 different concentration series of AGEs-BSA and AGEs-BSA control forl ,3,6 and 9 days, while blank control group was incubated without any intervention. Then bFGF expression in Müller cells was half-quantitatively detected by immunocytochemistry（ICC）. Results Comparing with blank control and AGEs-BSA control group,AGEs-BSA evoked a time and concentration-dependent increase of bFGF expression by retinal Müller cells. Conclusion AGEs might promote the neovascularization of diabetic retinopathy through up regulating the expression of bFGF in Müller cells.

关 键 词：糖基化终产物 碱性成纤维细胞生长因子 视网膜MÜLLER细胞 免疫细胞化学 

分 类 号：R774.1[医药卫生—眼科] R-33[医药卫生—临床医学]