DNA改组技术在酶工程中的应用  

Applications of DNA Shuffling in Enzyme Engineering

作　　者：刘莹[1] 曹健[1] 王中太[1] 杨永刚[1] 黄亮[1] 

机构地区：[1]河南工业大学生物工程学院,郑州450052

出　　处：《生物技术通报》2008年第3期54-56,共3页Biotechnology Bulletin

基　　金：河南省高校杰出科研人才创新工程项目(2006KYCX008);"河南省教育厅高等学校创新人才培养工程"(河南省教育厅;豫教高[2005]126号)

摘　　要：DNA改组技术是一种应用最广泛且简单有效的分子定向进化方法。它是一种高通量的随机突变筛选技术,可以对目的基因进行多次重组和选择,得到性质优良的突变文库。介绍了DNA改组技术的原理及其在酶工程中的应用。DNA shuffling is one of the most widely used methods as well as a simple and efficient technology for directed molecular evolution.DNA shuffling is a high throughput random mutation screening assay,which can recombinate and select the sequence of the target gene for many times,and mutation libraries of fine properties can be obtained as a result.Principle and application of DNA shuffling technique in the field of enzyme engineering was reviewed in this paper.

关 键 词：DNA改组 酶工程 应用 

分 类 号：Q814[生物学—生物工程]