人肝细胞癌总基因组DNA与c-myc、c-N-ras基因甲基化的改变  

Change of total genomic DNA methylation and the alternation of DNA methylation petterns of c-myc aud c-N-ras onco-genes in human hepatocellular carcinoma

作　　者：沈兰兰[1] 邱德凯[1] 房静远[1] 张腾飞[2] 杨甲梅 陈诗书[2] 萧树东[1] 

机构地区：[1]上海市消化疾病研究所,200001 [2]上海第二医科大学生化教研室,200001 [3]上海东方肝胆外科医院,200001

出　　处：《上海医学》1997年第10期559-561,共3页Shanghai Medical Journal

摘　　要：分别用两种方法研究了人原发性肝细胞癌总基因组及特定癌基因片段的DNA甲基化情况，并进行对比研究。以3H－SAM掺入法研究总基因组DNA甲基化水平，以限制性内切酶酶切、South－ern吸印法对c－myc、c－N－ras两种癌基因状况进行分析。结果：发现人肝细胞癌区和癌旁区组织内c－myc、c－N－ras癌基因分别有不同程度的低甲基化，且有癌区总基因DNA甲基化水平明显下降。本文结果提示：两种方法检测均发现在人原发性肝癌中存在DNA低甲基化现象，联用两种方法效果优于单一检测者。To study the total genomic DNA methylation level and the methylation patterns of some specific onco-genes in human hepatocellular carcinoma from two different aspects. The level of total genomic DNA methylation wasmeasured by incubating DNA with 3 H-S-adenosylmethionine (3 H-SAM ). The methylation patterns of c-myc and c-N-ras oncogenes were assayed by using Southern blotting technique and restriction enzymes(Hpa /Mspl). c-myc and c-N-ras oncogene fragments were hypomethylated to different degree in DNA samples derived from both cancerous (10/33and 20/33)and adjacent paracancerous (4/33 and 10/33)liver tissues respectively,and the level of total genomic DNAmethylation in cancerous tissues was significantly low. DNA hypometbylation was discovered in human primary livercancer. It is recommended to use both methods jointly instead of one singly.

关 键 词：肝细胞癌 DNA 甲基化 癌基因 肝肿瘤 

分 类 号：R735.702[医药卫生—肿瘤]