YH-16对宫颈癌细胞及其裸鼠移植瘤抑制作用研究  

Inhibition of YH-16 on growth of both Hela cells and Hela implanted tumor in nude mice

作　　者：程丽[1] 刘梅梅[2] 张云艳[1] 隋丽华[1] 

机构地区：[1]哈尔滨医科大学附属第三医院妇科,黑龙江哈尔滨150040 [2]哈尔滨医科大学附属第二医院妇产科,黑龙江哈尔滨150086

出　　处：《中国现代医学杂志》2008年第10期1324-1327,共4页China Journal of Modern Medicine

摘　　要：目的探讨YH-16(重组内皮抑素)对宫颈癌Hela细胞及其荷瘤鼠生长抑制作用。方法用MTT法检测细胞生长;用透射电镜观察细胞凋亡、流式细胞仪检测细胞凋亡率及细胞周期;将Hela细胞移植至裸鼠皮下,观察不同浓度YH-16(0、0.4、0.75和1.5mg/kg)对荷瘤鼠皮下移植瘤生长的影响;TUNEL法观察肿瘤细胞的凋亡;免疫组化方法检测裸鼠移植瘤组织中肿瘤微血管密度(MVD)。结果YH-16抑制Hela细胞增殖(P<0.01);能诱导Hela细胞凋亡。YH-16能抑制裸鼠皮下移植瘤的生长(P<0.05);YH-1615mg/kg组的肿瘤MVD计数较对照组和其他治疗组明显降低(P<0.05)。结论YH-16具有抑制宫颈癌Hela细胞及其皮下移植瘤生长的作用。[Objective] To evaluate the inhibitory effect of YH-16（rhEndostatin） on the growth of cervix cancer cell lines Hela and Hela implanted tumor in nude mice. [Methods] The effect of Endostatin on Hela cell proliferation was studied by means of MTT. The cell apoptosis was detected by transmission electron microscope and cell flow cytometry. The Hela cells were subcutaneously inoculated into nude mice. The therapeutic efficacy of different doses YH-16 （0, 0.4, 0.75 and 1.5 mg/kg） on bearing cancer mice was detected. The cell apoptosis of implanted tumor was detected by TUNEL stanning. [Results] YH-16 inhibited Hela cells proliferation （P 〈0.01） and induced cell apoptosis. YH-16 significantly suppressed the growth of the implanted rumor in nude mice（P 〈0.05）. The MVD in mice treated with YH-16 15mg/kg was much lower than other groups（P 〈0.05）. [Conclusions] Endostatin had inhibitory effects on the growth of both Hela cell and implanted tu mors in nude mice.

关 键 词：YH-16 宫颈肿瘤 异种移植模型抗肿瘤实验 肿瘤细胞 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]