大鼠坐骨神经分支选择结扎切断模型的建立及脊髓背脚N-甲基-D-天冬氨酸受体检测  

Effects of intrathecal butorphanol on rat′s pain threshold and changes of neuronal NMDA receptor expression in rat spinal dorsal horn following spared nerve injury of sciatic nerve

作　　者：魏小洁[1] 郭曲练[1] 王懿春[1] 李翔 

机构地区：[1]中南大学湘雅医院,湖南长沙410008 [2]湖南省武警医院麻醉科,湖南长沙410006

出　　处：《中国现代医学杂志》2008年第10期1383-1387,共5页China Journal of Modern Medicine

摘　　要：目的采用免疫组织化学方法,观察SNI模型大鼠脊髓背角神经元NMDAR的表达。方法健康雄性SD大鼠15只,随机分为3组:对照组(C1组)、假手术组(C2组)和生理盐水组(NS组),每组5只。C1组不做任何处理,其他2组均根据改良Yaksh法进行鞘内置管。置管5d后,NS组按Woolf等方法建立神经病理性疼痛模型(SNI),C2组除不损伤神经外处理同NS组;制模2d后,C2组和NS组用微量注射器鞘内注射20μL生理盐水,然后用生理盐水冲管(共20μL)。在30min后,C2组、NS组均进行疼痛行为学观察。3组均在注药后2h处死大鼠,用免疫组化法观察大鼠腰5节段水平脊髓背角NMDAR的表达。结果C1组和C2组在各时点均无机械性异常疼痛出现,热刺激后爪退缩潜伏时间差异也均无统计学意义(P>0.05);NS组在SNI手术后第1天和第2天出现明显的痛觉过敏(机械性异常疼痛痛阈降低),与C1组比较差异有统计学意义(P<0.01),但对热刺激的后爪退缩潜伏时间与C1组比较差异无统计学意义(P>0.05);NS组大鼠脊髓背角NMDAR免疫阳性细胞数量与C1及C2组比较明显增加,两者之间差异有统计学意义(P<0.05);NS组的脊髓背角NMDAR免疫阳性细胞数密度值与C1,C2组相比显著增高(P<0.05),NS组的阳性细胞光密度值较C1组和C2组增高(P<0.05)。结论SNI模型可引起大鼠损伤侧肢体机械性痛觉过敏,但对热刺激不敏感;SNI模型引起大鼠脊髓背角神经元NMDAR表达上调。[Objective] To observe the changes of neuronal NMDA receptor expression in rat spinal dorsal horn following spared nerve injury of sciatic nerve by using immunohistochemistry method. [Methods] Fifteen healthy male Sprague-Dawley rats （200-270 g） were randomly devided into three groups（5 in each group）： control group（group C1 and group C2）, normal saline group（group NS）. There weren′t operative procedure in group C1 rats. The rats in other two groups were was insterted intrathecally into the lumbar region（about 8 cm） according to the method of Yaksh. After 5 days, the rats in group NS were made into the spared nerve injury model （SNI）. Group C2 involved exposure of the sciatic nerve and its branches without any lesion; group C2, NS were intrathecally injected 20 μL normal saline or 10 μL Butorphanol （12 μg and 24 μg） followed by 10 μL normal saline after 2 days post-surgery, respectively. Mechanical allodynia and radiogenic heat pain thresholds were measured pre-surgery and on day 1, 2 post-surgery and 30 min after intrathecally injection. 2 hours after intrathecally injection, all rats were killed, and the L5 spinal cord were dissected for determination of the expression of NMDA receptor using immunohistochemical techniques. [Result] In group C1 and group C2, there were no difference on the statistics in pain threshold （P 〉0.05）. ②There were significant difference on the statistics in pain threshold in group NS and C1, C2 （P 〈0.01） on day 1,2 post-surgery, but the radiant heat stimulus paw withdrawal latency was no change in three groups compared with that in group C1, C2 （P 〉0.05）. The number of immunoreactive soma in the superficial laminae of the L5 spinal cord was significantly incressed significantly in group NS than that in group C1 and group C2（P 〈0.05）. ④The immunoreactive soma quantity density of NMDAR was higher in group NS than those in group C1 and C2 （P 〈0.05）, The immunoreactive soma optical density of NMDAR was higher in SNI mod

关 键 词：神经病理痛 脊髓 N-甲基-D-天冬氨酸受体 

分 类 号：R-33[医药卫生]