胃癌及慢性胃炎幽门螺杆菌的蛋白质组学研究  

Proteomic analysis of Helicobacter pylori in human gastritis and gastric cancer

作　　者：游运辉[1] 范学工[2] 刘萍[1] 刘洪波[2] 田雪飞[2] 颜雪梅[1] 

机构地区：[1]中南大学湘雅医院风湿免疫科,长沙410008 [2]中南大学湘雅医院感染病科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第5期384-390,共7页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(3027117)~~

摘　　要：目的:建立胃炎与胃癌相关幽门螺杆菌的双向凝胶电泳图谱,识别鉴定其差异表达的蛋白质,探讨细菌本身因素在发病过程中的作用。方法:利用固相pH梯度双向凝胶电泳分离胃炎与胃癌相关幽门螺杆菌菌株的总蛋白质,凝胶经蓝银显色后,采用PDQuest图像分析软件比较、分析、识别差异表达的蛋白质,应用基质辅助激光解吸电离飞行时间质谱得到相应的肽质指纹图谱,然后搜索数据库鉴定部分差异蛋白质点。结果:获得分辨率较高、重复性较好的胃炎与胃癌相关幽门螺杆菌双向凝胶电泳图谱,鉴定出14个差异蛋白质,包括抗氧化作用、分子伴侣、解毒和DNA修复相关蛋白质、三大代谢相关酶类、细胞结构相关蛋白质以及信号传导相关蛋白质。结论:建立了分辨率高且重复性较好的胃炎与胃癌相关幽门螺杆菌双向凝胶电泳图谱,并识别鉴定出了两种不同疾病来源幽门螺杆菌差异表达的蛋白质,其蛋白质表达的差异可能在胃癌的发生中起重要作用。Objective To establish 2-dimensional electrophoresis （ 2-DE ） maps of Helicobacter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins, and to discuss the role of bacterial factor in pathogenesis. Methods The total proteins of Helicobacter pylori in human gastritis and gastric cancer were separated by immobilized pH gradient-based 2-DE The differentially expressed proteins were screened by PDQuest analysis software and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry, and searched on database. Results A well-resolved and reproducible 2-DE pattern of Helicobacter pylori was obtained from patients with human gastritis and gastric cancer. Fourteen differentially expressed proteins were identified, including proteins related to anti-oxidation, molecular chaperones and detoxification, enzymes related to metabolism, proteins related to cytoarchitecture, and proteins related to signal conduction. Conclusion A well-resolved and reproducible 2-DE pattern of Helicobacter pylori in human gastritis and gastric cancer is established and differentially expressed proteins from these 2 diseases are identified. The differentiation of protein expression may play an important role in the pathogenesis of gastric cancer.

关 键 词：幽门螺杆菌 蛋白质组 胃癌 基质辅助激光解吸电离飞行时间质谱 

分 类 号：R735.2[医药卫生—肿瘤] R573.3[医药卫生—临床医学]