Cystatin M在乳腺癌及转移癌中的表达和临床意义  

Expression of cystatin M in breast cancer and metastatic cancer and its clinical significance

作　　者：王欣[1] 闫哲[1] 李晓青[2] 冯玉梅[2] 曹旭晨[1] 

机构地区：[1]天津医科大学附属肿瘤医院乳腺一科 [2]天津医科大学附属肿瘤医院生化及分子生物研究室

出　　处：《中华乳腺病杂志（电子版）》2008年第3期21-25,共5页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：天津医科大学肿瘤医院人才启动基金(编号6-28)资助

摘　　要：目的探讨cystatin M在乳腺癌及转移癌中的表达和临床意义。方法采用RT-PCR检测108例乳腺癌标本、30例转移癌标本及24例癌旁正常乳腺组织中cystatin M mRNA表达水平,分析cystatin M基因表达与临床病理参数的关系。结果乳腺癌标本中cystatin M在Ⅰ、Ⅱ期乳腺癌患者中表达较高,而在III/IV期乳腺癌患者中表达较低,两者间差异有统计学意义;cystatin M在5cm以下肿瘤中表达较高,而在5cm以上肿瘤中表达较低,两者间差异有统计学意义;乳腺癌标本中cystatin M表达量与正常乳腺组织的差异无统计学意义;乳腺癌标本中cystatin M表达量与转移癌标本的差异也无统计学意义;乳腺癌标本中cystatin M mRNA表达水平与乳腺癌患者是否发生淋巴结转移、淋巴结转移数、组织学分级、病理学类型无关;乳腺癌标本和转移癌标本中cystatin M mRNA表达水平与人表皮生长因子受体2状态,雌激素受体状态及孕激素受体状态无关。结论Cystatin M是否为监测乳腺癌浸润转移的可靠指标仍需探讨。Objective To explore the expression of cystatin M in breast cancer and its clinical significance. Methods RT-PCR was used to measure the level of cystatin M mRNA in 108 samples of breast cancer, 30 samples of metastatic cancer and 24 samples of normal breast tissues. The relationship between the expression of cystatin M gene in human breast cancer and clinilcopathological features was analyzed. Results The cystatin M expression level of breast cancer samples was high in stage Ⅰ/Ⅱbreast cancer patients, but low in stage Ⅲ/Ⅳ breast cancer patients, and there was a statistical significance between them. The systatin expression was high in 〈 5 em tumors, but low in 〉 5 cm tumors, with a statistical significance between them. Cystatin M expression levels of breast cancer were correlated with TNM staging and clinical size of tumor. The expression levels of cystatin M in the breast cancer tissue were not significantly different from normal breast tissues and metastatic cancer tissues. Cystatin M expression levels of breast cancer were not correlated with lymph node metastasis, axillary lymph node status, histological grade or pathological type. Cystatin M expression levels of breast cancer and metastatic cancer were not correlated with HER-2, ER or PR status. Conclusion It needs to be discussed whether cystatin M can be a prognostic marker for breast cancer metastasis.

关 键 词：乳腺癌 CYSTATIN M MRNA表达 转移 

分 类 号：R737.9[医药卫生—肿瘤]