Bcl-2基因在口腔上皮癌多药耐药细胞的表达  

Expression of Bcl-2 in KBV cell

作　　者：董岩[1] 

机构地区：[1]大连医科大学口腔医学院,辽宁大连116044

出　　处：《大连医科大学学报》2008年第3期218-220,共3页Journal of Dalian Medical University

摘　　要：[目的]通过检测Bcl-2基因在口腔上皮癌敏感细胞株(KB)与多药耐药细胞株(KBV)的表达差异,探讨Bcl-2基因在口腔上皮癌细胞多药耐药性发生中的作用。[方法]体外培养口腔上皮癌多药耐药株及敏感株,通过RT-PCR法及免疫荧光法检测Bcl-2基因在KB及KBV细胞中转录及翻译水平的差异,通过MTT法及流式细胞检测技术检测KB及KBV细胞对长春新碱(VCR)敏感度的差异。[结果]免疫荧光图像显示Bcl-2蛋白在KB细胞中弱表达,而在KBV细胞中强表达;RT-PCR检测结果显示,KB细胞Bcl-2/β-actin比值是25.0%,KBV组Bcl-2/β-actin比值为36.1%,两组细胞间差异具有统计学意义(P<0.01);MTT法检测结果显示KBV组细胞对长春新碱的耐药性为KB组的16.5倍,流式细胞仪检测结果显示KB细胞凋亡率达(98.8±1.2)%;KBV细胞凋亡率达(23.5±2.1)%,两组间差异具有统计学意义(P<0.01)。[结论]Bcl-2基因与口腔上皮癌耐药性的发生密切相关。[ Obejective ] To investigate the effect of Bcl - 2 on development of the muhidrug resistance in KBV cells, [ Methods ] RT- PCR and immunofluorescent method were used to detect the Bcl- 2 expression at the mRNA and protein levels in KB and KBV cells. MTT and flow cytometry（FCM） were used to detect the chemosensitivity to VCR in KB and KBV cells. [ Results ] The immunofluorescent image in KBV cells was stronger than that in KB cells at the Bcl - 2 protein level. At the mRNA level, Bcl- 2/β -actin was 36, 1% in KBV cells and 25.0% in KB cells ,P 〈 0.01. The apoptosis rates in KB and KBV cells were （98.8 ± 1.2）% and cells （23.5 ± 2.1 ）% respectively, P 〈 0.01. [ Conclusions ] This result suggested that Bcl -2 might play an important role in development of muhidrug resistance of KBV cells.

关 键 词：KBV/KB BCL-2 多药耐药性 

分 类 号：R739.8[医药卫生—肿瘤]