NDRG-1基因甲基化与乳腺癌发生的关系  

Hypermethylation of CpG island in the promoter region of NDRG-1 gene in breast cancer

作　　者：王立玮[1] 何杰[1] 周东蕊[2] 房汝敬[2] 张仁敏[2] 陆祖宏[2] 

机构地区：[1]东南大学附属中大医院病理科,南京210009 [2]东南大学分子电子学国家重点实验室,南京210096

出　　处：《临床与实验病理学杂志》2008年第2期146-150,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：江苏省卫生厅重点科研课题(H200543)

摘　　要：目的对NDRG-1基因启动子区甲基化状态进行研究,探讨NDRG-1基因甲基化在乳腺癌发生中的作用。方法采用双色荧光杂交微阵列基因芯片技术对33例乳腺癌样本和癌旁组织样本进行NDRG-1基因启动子区甲基化状态研究。结果33例肿瘤样本和癌旁组织样本中NDRG-1基因启动子区CpG位点均有不同程度的甲基化,肿瘤组织中NDRG-1基因启动子区CpG位点甲基化率显著高于相应的癌旁样本组织(t=14.12,P<0.05)。结论DNA甲基化作为NDRG-1基因的重要调控机制,NDRG-1基因启动子区过甲基化可能在乳腺癌的发生过程中起重要作用。Purpose To explore the correlations of methylation status in the promoter region of NDRG-1 gene to carcinogenesis of breast cancer. Methods Microarray-based methylation analysis using dual-color fluorescence hybridization was used to detect the methylation status in the promoter region of NDRG-1 gene in 33 pairs of breast cancer and corresponding normal tissues. Results . The methylation level of CpG island in the promoter region of NDRG-1 gene in breast cancer was higher than in tissues adjacent（ t = 14. 12, P 〈 0.05）. Conclusions As important regulation and control mechanism of NDRG-1 gene, hyper-methylation of CpG island in the promoter region of NDRG-1 gene possibly plays an important role in carcinogenesis of breast cancer.

关 键 词：乳腺肿瘤 甲基化 NDRG-1基因 

分 类 号：R737.9[医药卫生—肿瘤]