乳腺癌VEGF、MMP-9及COX-2蛋白表达与淋巴道转移和血管生成的相关性  

Relationship between expressions of VEGF,MMP-9,COX-2 and lymph node metastasis and angiogenesis in breast carcinoma

作　　者：王丽辉[1] 王波[1] 李连宏[1] 湛丽[2] 张众[1] 

机构地区：[1]大连医科大学病理学教研室,大连116044 [2]大连市妇产医院病理科,大连116012

出　　处：《临床与实验病理学杂志》2008年第2期154-157,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：辽宁省科技厅自然科学基金项目(20042136)

摘　　要：目的通过检测血管内皮细胞生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)、环氧化酶-2(COX-2)在乳腺癌细胞中的表达情况来探讨它们与乳腺癌淋巴结转移和微血管密度(MVD)的关系。方法采用免疫组化SP法检测74例乳腺浸润癌(有淋巴结转移者39例,无淋巴结转移者35例)中VEGF、MMP-9、COX-2和CD34的表达,并用多因素Cox比例风险模型分析患者的预后。结果VEGF、MMP-9、COX-2的表达与MVD值在淋巴结转移组与无转移组之间的差异均具有显著性(P<0.05),与乳腺癌淋巴结转移呈正相关;VEGF、MMP-9、COX-2蛋白表达与MVD值呈正相关(P<0.05);COX-2的表达随着乳腺癌病理分级的增高而增强;MVD值高者生存时间短。结论乳腺癌VEGF、MMP-9、COX-2蛋白表达与其淋巴道转移和MVD有关,检测这几种蛋白表达将有助于判断乳腺癌的转移潜能、血管生成能力及预后。Purpose To explore the expressions of cyclooxygenase-2 （COX-2）, matrix metalloproteinase-9 （MMP-9）, vascular endothelial cell growth factor（VEGF） in breast carcinoma tissue and their associations with lymph node metastasis and microvascular density （MVD）. Methods The expressions of VEGF, MMP-9, COX-2 and CD34 were examined in 74 cases of breast carcinoma including 39 cases with lymph node metastasis and 35 cases without lymph node metastasis by immunohistochemistry. The patient＇ s prognosis was analyzed by muhivariable Cox proportional hazards model. Results The expressions of MMP-9, VEGF, COX-2 and MVD in lymph node metastasis group were significantly higher than that in no lymph node metastasis group （ P 〈 0. 05 ）. The expressions of VEGF, MMP-9, COX-2 were correlated with the average of MVD（ P 〈 0.05 ）. The expression of COX-2 was related with the histological grade of breast carcinoma. Patients with low average of MVD group had longer survival time. Conclusions The expressions of VEGF, MMP-9 and COX-2 are strongly associated with lymph node metastasis and MVD of breast carcinoma. These proteins are indicators of metastasis and predictors for the prognosis of breast carcinoma.

关 键 词：乳腺肿瘤 淋巴道转移 血管内皮细胞生长因子 基质金属蛋白酶-9 环氧化酶-2 微血管密度 

分 类 号：R737.9[医药卫生—肿瘤]