MUM1蛋白在弥漫性大B细胞淋巴瘤中的表达及意义  

MUM1 protein expression and its clinicopathologic significance in diffuse large B-cell lymphomas

作　　者：褚红娟[1] 宋兰英[2] 蒋会勇[1] 陈愉 赵彤[2] 

机构地区：[1]南方医科大学广东省分子肿瘤病理重点实验室,广州510515 [2]南方医科大学附属南方医院病理科,广州510515

出　　处：《临床与实验病理学杂志》2008年第2期207-210,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：广东省社会发展攻关项目(B30301);广州市科技计划项目(2002Z34061)

摘　　要：目的探讨弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中活化的B细胞相关蛋白MUM1的表达与临床病理特征之间的关系。方法利用组织微阵列免疫组化检测60例DLBCL石蜡包埋组织中MUM1、bcl-6和CD10的表达。结果60例DLBCL被分为两种抗原表达表型:一种为活化的B细胞表型(A型)表达MUM1;另一种为生发中心B细胞表型(B型),表达CD10和(或)bcl-6但不表达MUM1。60例DLBCL中61.67%为A型,31.67%为B型,其中59.25%中心母细胞型,3/4免疫母细胞型,2/2间变性大B细胞型均为A型。A型在结外和结内DLBCL中分别占61.76%和61.54%,而在胃肠道DLBCL中的比例(47%)显著低于在其他结外DLBCL中的比例(80%,P=0.079)。在MUM-1(+)/bcl-6(+)/CD10(+/-)的病例中,75.00%(12/16)为结外DLBCL,高于在MUM-1(+)/bcl-6(-)/CD10(-)病例中的比例43.75%(7/16),但差异没有显著性(P=0.149)。结论A型(表达MUM1)在DLBCL中的比例较高,提示MUM1表达很可能与DLBCL组织学变异有关,联合检测CD10和bcl-6,可协助DLBCL分型诊断。Purpose To investigate the correlation between activated B pattern of MUM1 protein expression and the main clinicopathological features in diffuse large B-cell lymphoma （DLBCL）. Methods Immunohistochemical staining for MUM1, bcl-6, and CD10 was performed on paraffin-embedded tissue microarrays from 60 cases of DLBCLs. Results The cases were classified into two antigenic expression patterns： activated B-cell pattern （pattern A） expressing MUM1, and germinal center B-cell pattern （pattern B） expressing CD10 and/or bcl-6 but not MUM1. 61.67% DLBCLs showed pattern A, and 31.67% pattern B. 59. 25% Centroblastic, 3/4 immunoblastic and 2/2 anaplastic variants were pattern A. Pattern A was observed in 61.76 % extranodal and 61.54% nodal DLBCLs, and significantly lower in gastrointestinal DLBCL （47%） than in other extranodal DLBCL （80% , P =0. 079）. In MUM1 （ ＋ ）/bcl- 6（ ＋ ）/CD10 （ ＋/- ） cases, 75.00% （12/16） cases were extranodal DLBCLs, higher than 43.75% （7/16） in MUM-1 （ ＋ ）/bcl- 6 （ - ）/CD10 （ - ） cases, but with no significant difference （ Fisher＇ s exact test P = 0. 149）. Conclusions The proportion of pattern A （MUM1 expression） is high in DLBCL, indicating that MUM1 expression is probably related to histological variants, and the detection of bcl-6 and CD10 coxpression can help in subtyping of DLBCL.

关 键 词：淋巴瘤 B细胞 MUM1蛋白 活化的B细胞表型 免疫组化 组织微阵列 

分 类 号：R733.4[医药卫生—肿瘤]