基质金属蛋白酶血管内皮生长因子与兔角膜新生血管生长与退化的相关  

Experimental Study on Correlation of Matrix Metalloprteinases and VEGF with the Growth and Regression of Neovascularization in the Rabbit Cornea

作　　者：杜红艳[1] 阎晓红[2] 闫元奎[3] 

机构地区：[1]内蒙古自治区医院眼科,内蒙古呼和浩特010017 [2]内蒙古医学院细胞化学教研室,内蒙古呼和浩特010059 [3]内蒙古医学院附属医院眼科,内蒙古呼和浩特010050

出　　处：《内蒙古医学杂志》2008年第5期522-525,共4页Inner Mongolia Medical Journal

摘　　要：目的:观察兔角膜新生血管的生长及退化过程中,基质金属蛋白酶、血管内皮生长因子的表达。方法:将45只健康新西兰大白兔随机取5只,为正常对照组,余40只作角膜缝线,建立角膜新生血管动物模型,随机分为阳性对照组和200μg/ml的GM6001用药组,用免疫组化及图像分析来研究两组的VEGF、MMP-9的表达和变化,并进行微血管计数。结果:在术后不同时间点,GM6001用药组MMP-9平均积分光密度(MMP-9 IOD)、VEGF IOD均较阳性对照组明显减少,差异有显著意义(P<0.001);VEGF IOD、MMP-9 IOD、微血管计数间有高度相关性(r=0.9840,r=0.7260)。结论:VEGF、MMP-9表达与角膜新生血管的形成与退化平行。GM6001可抑制和延迟实验性CRNV的形成。Objective： To observe the expression of matrix metalloproteinase and Vascular Endothelial Growth during the develop and degenerate of neovascularization in the rabbit cornea. Methods： The model of cornea new vessel was induced by suturing cornea. 5rabbits （ten eyes） from forty- five rabbits were randomly used as normal control. The others received suturing cornea, which were randomly divided into positive control group and GM6001 group . Immunohistochemistry analysis, technique and computerizeimage analysis were used to determine the expression and changes of VEGF, MMP-9, accounting microvessels respectively. Results：At rhe different periods, MMP-9 IOD, VEGF IOD and microvessel in positive control group were significantly lower than that in GM600I control group （P〈0. 001）, and then exisiting significant correlativity（ r = 0. 9840, r = 0. 7260）. Concluson： The expression of MMP-9 and VEGF are parallel with corneal neovascularization. GM6001 can prevent and delay the corneal neovascularization.

关 键 词：基质金属蛋白酶 血管内皮生长因子 角膜新生血管 

分 类 号：R772[医药卫生—眼科]