非小细胞肺癌组织中p33^(ING1b) mRNA表达水平及生物学意义  

Expression Level and Biological Significance of p33^(ING1b) mRNA in Non-small Cell Lung Cancer

作　　者：金军[1] 叶亚平[1] 胡国成[1] 

机构地区：[1]湖北省鄂州市中心医院肿瘤放疗科,湖北鄂州436000

出　　处：《海南医学》2008年第7期33-35,共3页Hainan Medical Journal

摘　　要：目的探讨p33ING1b mRNA的表达水平与非小细胞肺癌(Non-small Cell Lung Cancer,NSCLC)临床病理特征的关系及其在NSCLC发生发展中的可能作用及生物学意义。方法用逆转录聚合酶链反应(RT-PCR)检测58例NSCLC患者中的癌组织及其癌旁组织p33ING1b mRNA表达水平。结果NSCLC组织中p33ING1b mRNA表达强度(0.408±0.156)低于癌旁组织(0.601±0.326),两者比较差异有统计学意义(P<0.05)。NSCLC组织中p33ING1b mRNA表达水平与病理分级、临床分期、淋巴结转移有密切关系(P<0.05),而与年龄、性别、吸烟与否、组织学类型无关(p>0.05)。结论p33ING1b mRNA低水平表达在NSCLC发生发展过程中起重要作用,P33ING1b低水平表达可作为一个预测NSCLC转移潜能以及不良预后的生物学指标。Objective To explore the relationship between p33^ING1b mRNA Expression Level and clinico- pathologic characterstics in Non-small Cell Lung Cancer （NSCLC） and the possible mechanism of p33^ING1b in the oncogenesis of NSCLC. Methods Reverse transcriptase-polymemseehain reaction （RT-PCR） method was employed to detect the mRNA level of p33^ING1b in NSCLC tissues and adjacent tissues of cancer. Results The expression level of p33^ING1b mRNA in NSCLC tissues （0.408±0.156） was significantly lower than that in adjacent tissues of cancer （0.601±0.326） （P〈0.05）. The level of p33^ING1b mRNA was significantly associated with pathologi- cal grades, clinical stages and lymph node metastasis, but not associated with sex, age, smoking and tissue types （p〉0.05）. Conclusion The decrease of p33^ING1b mRNA may play an important role in the tumorigenesis and development of NSCLC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.

关 键 词：肺肿瘤 逆转录聚合酶链反应 P33^ING1B 基因 

分 类 号：R734.2[医药卫生—肿瘤]