小剂量环磷酰胺治愈荷膀胱癌小鼠过程中外周血血小板计数的动态变化及意义  

Changes and significance of peripheral blood platelet count in tumor shrinkage induced by a low dose of CTX in T739 mice

作　　者：李墨林[1] 贾玉杰[1] 姜妙娜[1] 舒晓宏[2] 李传刚[3] 

机构地区：[1]大连医科大学基础医学院病理生理教研室,辽宁大连116044 [2]大连医科大学药学院,辽宁大连116044 [3]大连医科大学附属二院泌尿外科,辽宁大连116044

出　　处：《细胞与分子免疫学杂志》2008年第6期567-569,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：辽宁省自然科学基金资助项目(20062156)

摘　　要：目的:建立环磷酰胺(CTX)治愈荷膀胱癌T739小鼠的动物模型,观察外周血象特别是血小板计数在化疗治愈肿瘤中的动态变化及其意义。方法:给正常T739小鼠皮下接种小鼠可移植性膀胱移行细胞癌组织,建立荷膀胱癌的小鼠模型。接种后第7天,荷瘤小鼠腹腔内分别注入15、40、100mg/kg的CTX,等量生理盐水对照,确定CTX治愈肿瘤的量效关系。然后再取12只荷瘤小鼠,随机分成2组,15mg/kgCTX单次腹腔内注射,等量生理盐水对照,分别在用药后6h、用药后第2、4、9、14天内眦静脉取血,进行血常规检测,分析荷瘤小鼠外周血象的动态变化。结果:15～100mg/kgCTX治疗后2周内,荷瘤小鼠肿瘤生长受抑制或肿瘤结节缩小的速率与所用CTX的剂量呈正相关;CTX治疗后2月,荷瘤小鼠的存活率与所用CTX的剂量呈负相关。15mg/kgCTX单次腹腔内注射在治愈多数荷瘤小鼠的同时对外周血象无明显抑制作用。用药后6h治疗组荷瘤鼠外周血血小板计数为(1483.4±184.4)×109/L,明显高于对照组小鼠(1086.6±81.0)×109/L,两组数据比较具有统计学意义(P<0.01);用药后第2、4、9、14天治疗组荷瘤鼠外周血血小板计数与对照组小鼠比较差异无统计学意义(P>0.05)。结论:15mg/kgCTX可治愈多数荷膀胱癌T739小鼠。单剂量CTX治疗后6h荷瘤小鼠外周血血小板计数的一过性增高可能与其抗肿瘤作用有关。AIM. To establish a mouse model for BTT739 tumor-bearing mice cured by a low dose of cyclophosphamide （CTX）, And then to observe the dynamic changes and significance of peripheral blood counts especially blood platelet count during tumor shrinkage induced by a low dose of CTX in T739 mice, METHODS： Mouse bladder carcinoma tissues were inoculated subcutaneously into T739 mice, Seven days later, different doses of CTX or the same volume of NS were administered intraperitoneally to treat these tuomr-bearing T739 mice. Tumor sizes were observed and recorded subsequently to find out the minimal dose of CTX that could cure most of these tumor-bearing mice, Then another 12 tumor-bearing mice were randomly divided into 15 mg/kg CTX treatment group and control group, Blood samples were obtained from orbital venous sinus on different times after CTX treatment. Complete blood counts were performed and the relationship between peripheral blood platelet counts and tumor shrinkage was analyzed. RESULTS： Within 2 weeks after CTX treatment, the speed of tumor shrinkage had a positive relationship with the dose of CTX used; but the survival rate of the tumor-bearing mice had a negative relationship with the dose of CTX used in 2 months after CTX treatment. 15 mg/kg CTX could cure most of the tumor bearing mice, while it had no remarkably inhibitive effects on peripheral blood cells. The perpherial platelet count increased to （ 1483. 4 ± 184. 4） × 10^9/L in mice 6 h after CTX treatment, There was significant difference compared with that in mice of control group （1086.6±81.0） ×10^9/L （ P 〈 0, 01 ）, During the 2nd to 14th day after CTX treatment, there was no obvious difference in the platelet count between treatment group and control group （ P 〉 0, 05）, CONCLUSION： CTX 15 mg/kg could cure most of bladder tumor-bearing 1-739 mice, The transient increase of the peripheral platelet count in 6 h after CTX treatment may relate to the antitumor effects of CTX.

关 键 词：环磷酰胺 肿瘤治愈 膀胱癌 T739小鼠 血小板计数 

分 类 号：R730.5[医药卫生—肿瘤]