1,3-丙二醇发酵过程中盐浓度的胁迫作用  

1,3-Propanediol Production Under Salt Stress

作　　者：徐佳杰[1] 刘朋波[1] 陶春平[1] 程骋[1] 付水林[1] 宫衡[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物工程学报》2008年第6期1098-1102,共5页Chinese Journal of Biotechnology

摘　　要：通过对克雷伯氏菌(Klebsiella pneumoniae)甘油发酵生产1,3-丙二醇(1,3-PD)过程的研究发现,盐浓度对1,3-PD发酵有胁迫作用。盐浓度较低时,菌体生长和产物生成均维持较高速率;盐浓度较高时会导致菌体生长减慢,1,3-PD最终浓度,甘油到1,3-丙二醇的转化率降低,同时1,3-丙二醇氧化还原酶受到抑制。在5m3罐中控制合适的盐浓度可以提高1,3-PD的发酵水平,使1,3-PD的最终浓度达到64g/L,转化率61%,生产强度2.1g/(L·h)。Through studying the process of glycerol fermentation to 1, 3-propanediol（1, 3-PD） by Klebsiella pneumoniae, it was found that the cell growth and product （or by-product） production were under salt stress. Cell growth and product formation kept high rate at low salt concentration. High salt concentration led to low growth of cells, final concentration of 1, 3-PD and conversion from glycerol to 1, 3-PD, and, 1, 3-propanediol oxidoreductase activity decreased. When the salt concentration in 5 m^3 bioreactor was controlled under appropriate manner, the concentration of 1, 3-PD production was markedly enhanced. The final 1, 3-PD concentration ,the conversion of glycerol to 1, 3-PD and productivity were 64 g/L, 61% and 2.1 g/（L · h）.

关 键 词：盐浓度 中试实验 发酵条件 氧化还原电位 

分 类 号：TQ223.162[化学工程—有机化工]