腹水中可溶性CD44v6和腺苷腹氨酶检测及意义  

Detection of sCD44v6 and ADA in malignant ascites

作　　者：马志敏 杨静华[2] 王运端 董晓瑜[3] 冯莉 

机构地区：[1]河北省石家庄市第四院妇产科,050011 [2]河北省胸科医院妇产科 [3]河北省石家庄市第二医院妇产科

出　　处：《河北医药》2008年第5期614-615,共2页Hebei Medical Journal

基　　金：河北省科技支撑计划项目(编号:072761864)

摘　　要：目的探讨可溶性CD44v6(sCD44v6)及腺苷腹氨酶(ADA)对良、恶性腹水的诊断价值。方法取肝硬化腹水20份、结核性腹水38份、恶性腹水30份,分别用酶联免疫吸附试验(ELISA)及酶速率法检测腹水sCD44v6及ADA水平。结果恶性腹水组sCD44v6水平为(102±30)ng/ml,明显高于良性腹水组(51±14)ng/ml(P<0.01)。肝硬化腹水组sCD44v6水平(55±12)ng/ml及结核腹水组cCD44v6水平(42±16)ng/ml,差异无统计学意义(P>0.05)。结核性腹水组ADA水平为(54±21)U/L,明显高于恶性性腹水组(21±12)U/L(P<0.01)。以0.5ng/ml为阳性界值,sCD44v6诊断恶性腹水的敏感性为83.3%、特异性为89.5%;以45U/L为阳性界值,ADA诊断结核性腹水的敏感性为86.8%、特异性为93.3%。结论sCD44v6和ADA对于良、恶性腹水的鉴别诊断有重要价值。Objective To explore the diagnostic value of sCD44v6 and ADA in benign and malignant ascites. Methods Total of 88 samples were collected and divided into 3 experimental groups： cirrhotic ascites （ n = 20） , tuberculous ascites （ n = 38）and malignant diseases （ n = 30）. sCD44v6 concentrations and ADA isoenzymc activity in all the specimens were determined by ELISA and enzyme kinetic analytical method respectively. Results sCD44v6 levels in malignant ascites were （101.6 ± 29.5） ng/ml, which was significantly higher than those in cirrhotic ascites（54.9 ± 12.4） ng/ml and tuberculous ascites （42.3 ± 16.8）ng/ml. However , there was no significant difference of sCD44v6 levels in cirrhotic ascites and tuberculous ascites （ P 〉 0.05）. ADA levels in tuberculous ascites were （54.1 ± 20.5） U/L, which was significantly higher than those in malignant ascites （21.2 ± 11.6） U/L（ P 〈 0.01 ）. Using 60.5 ng/ml as a cutoff limit, the sensitivity and specificity of sCD44v6 to diagnose malignant ascites were 83.3 % and 89.5% respectively. Using 45u/L as a cutoff limit , the sensitivity and specificity of ADA to diagnose tuberculous ascites were 86.8 % and 93.3% respectively. Conclusion The detection of sCD44v6 and ADA levels is useful in diagnosis of benign and malignant ascites.

关 键 词：腹水 可溶性CD44V6 ADA 

分 类 号：R735.2[医药卫生—肿瘤] R442.5[医药卫生—临床医学]