非小细胞肺癌p16基因甲基化及其临床诊断意义  

Diagnostic value of methylation of p16 genes in patients with non- small cell lung cancer

作　　者：薛青[1] 薛绍礼[2] 金永堂[2] 于在诚[4] 汪亚松[3] 陶文虎[4] 

机构地区：[1]首都医科大学宣武医院神经内科,北京100053 [2]安徽医科大学医学生物工程教研室 [3]浙江大学医学院 [4]安徽医科大学第一附属医院

出　　处：《中国综合临床》2008年第6期521-523,共3页Clinical Medicine of China

基　　金：国家自然科学基金（30471427）;安徽省重点科研项目（07021017）

摘　　要：目的研究非小细胞肺癌癌组织p16基因甲基化及其临床诊断意义。方法选择47例非小细胞肺癌患者，用MSP技术检测肺癌组织和癌旁正常组织p16基因甲基化。结果肺癌组织p16基因甲基化率44．7％，高于癌旁组织的17．0％（P〈0．01）；肺癌组织甲基化检出率与临床分期、临床病理分型和临床分类之间差异无统计学意义（均P〉0．05）。结论提示肺癌有关组织或样本的p16基因甲基化，可能成为各种临床类型的非小细胞肺癌早期诊断的一个有效指标。但特异性、灵敏性和可行性等有待进一步研究。Objective To detect rnethylation of p16 gene in lung cancer tissues of non- small cell lung cancer patients, and to approach its clinical diagnostic value. Methods The methylation of p16 gene in DNA from 47 non- small cell lung cancer tissues and corresponding nomalignant tissues were tested with methylation-specific PCR（ MSP）. Results The total frequency of p16 methylation was significantly higher in lung cancer tissues than that in the corresponding malignant tissue（44.7% vs 17% ） （P 〈 0.01）. But there was no significant difference in detectiveness, clinical staging, clinical pathology type and clinical classification （ P 〉 0.05 ）. Condusion The detection of methylation of p16 gene may be helpful to clinical diagnosis for non- small cell lung cancer, but its specify, sensitivity and feasibility need to be further studied.

关 键 词：非小细胞肺癌 P16基因 甲基化 诊断意义 

分 类 号：R734.2[医药卫生—肿瘤]