不同基肥对黄瓜根际土壤微生物群落多样性的影响  

Effect of different base fertilizers on soil microbial community diversity in cucumber rhizosphere

作　　者：吴凤芝[1] 于高波[1] 刘博[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《植物营养与肥料学报》2008年第3期576-580,共5页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目(30571264)资助。

摘　　要：分别以RAPD分子生物学方法和BIOLOG生理学方法,研究了不同基肥对黄瓜根际土壤微生物群落DNA序列多样性和群落功能多样性的影响。结果表明,在本试验条件下,基肥为75000kg/hm^2有机肥处理和75000kg/hm^2有机肥加300kg/hm^2复合肥处理最好;基肥为600kg/hm^2复合肥处理使土壤微生物群落DNA序列丰富度指数和多样性指数显著下降,与对照的DNA序列相似系数最低;有机肥处理有利于土壤微生物群落DNA序列多样性、均匀度和黄瓜产量的提高。此外,不同基肥处理改变了土壤微生物对单一碳源的利用能力。Genetic diversity and functional diversity of soil microbial community in cucumber rhizosphere as affected by different base fertilizers were studied by the Random Amplified Polymorphic DNA （RAPD） method and the BIOLOG technology, respectively. The results indicated that organic fertilizer at a rate of 75000 kg/ha or organic fertilizer （75000 kg/ha） with compound fertilizer at a rate of 300 kg/ha had higher effects on genetic diversity of soil microbial community under field conditions. Treatment of fertilizers as base application at level of 600 kg/ha resulted in decrease of DNA sequence richness index and diversity index of soil microbial communities, and the similarity coefficient of DNA sequence from soil microbial communities was the lowest compared with control. Organic fertilizer treatment was conducive to increasing DNA sequence diversity index and evenness index of soil microbial communities. Moreover, multivariate analysis of BIOLOG data indicated that organic fertilizer had a significant impact on sole carbon utilization pattern.

关 键 词：基肥 黄瓜 土壤微生物 RAPD BIOLOG 

分 类 号：S642.2[农业科学—蔬菜学]