一株产漆酶菌株的筛选鉴定和发酵条件的研究  

Studies on screening,identification and fermentation condition of a laccase-producing strain

作　　者：刘敏[1] 张明[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《生物学杂志》2008年第3期40-43,共4页Journal of Biology

基　　金：安徽省高校"十五"优秀人才计划(【教秘人】2004-100)

摘　　要：以愈创木酚为底物，采用平板筛选法筛选得到一株产漆酶菌株WS1-2，形态学特性表明该菌属于绿色木霉。对产酶条件的初步研究结果表明，WS1-2菌株的产酶高峰期出现在接种培养后的第4d。与蔗糖、乳糖、半乳糖和可溶性淀粉相比，以葡萄糖为碳源时，发酵上清液的漆酶活力明显要高，最大值达230U／L。以NH4Cl为氮源，最有利于WS1-2漆酶的产生，漆酶活力最高可达到234U／L。0.01mmol／L的愈创木酚和ABTS对WS1-2产漆酶有明显的诱导作用，3～5mg／L的Tween-80可以明显提高WS1—2的产酶水平。A laccase-producing WS1-2 was obtained from the medium with guaiacol substrate. It was identified as Trichoderma viride by its morphological features. Its peak of laccase production came out after incubated 4 days. Compared with the other carbon sources like sucrose, lactose, galactose and soluble starch, it showed to get the highest laccase activity up to 230U/mL by glucose. Meanwhile NH4Cl was the optimal nitrogen source, it could make the activity to 234U/mL. Guaiacol as well as ABTS in a concentration of 0. 01 mmol/L had evidently inductive effect for producing laccase by WS1-2, and Tween-80 could increase WS1-2 laccse-producing level in a concentration range of 3-5mg/L.

关 键 词：绿色木霉 愈创木酚 形态学特征 酶活力 

分 类 号：Q935[生物学—微生物学]