丁酸钠联合阿霉素对HeLa细胞端粒酶活性的影响  

Effects of Adriamycin Combined with Soidum Butyrate on Telomerase Activity in HeLa Cells

作　　者：孔虹[1] 梁彬[2] 林伟山 于成国[1] 

机构地区：[1]中国医科大学实验技术中心二部 [2]中国医科大学高等职业技术学院,沈阳110001 [3]营口经济技术开发区中心医院检验科,辽宁营口115007

出　　处：《中国医科大学学报》2008年第3期346-348,共3页Journal of China Medical University

摘　　要：目的研究阿霉素(ADR)与丁酸钠(NaBu)联用对HeLa细胞增殖和端粒酶活性的影响。方法MTT法检测NaBu、ADR以及两药联用的药效动力学特征;TRAP-银染法检测端粒酶活性;RT-PCR法检测hTERT mRNA表达。结果NaBu、ADR以及两药联用均可抑制HeLa细胞生长,ADR单独作用HeLa细胞48h的IC50为6000nmol/L,与1mmol/L的NaBu联用后降为320nmol/L,两组均呈剂量依赖性。ADR组和NaBu+ADR组的端粒酶活性随ADR浓度增加而降低,呈剂量依赖性,在ADR浓度为100nmol/L时差异有统计学意义(P<0.05);hTERT mRNA表达与端粒酶的变化趋势一致,在ADR浓度为10nmol/L、100nmol/L浓度时差异有统计学意义(P<0.05)。结论NaBu和ADR均可抑制HeLa细胞增殖,联合应用具有协同效应,与抑制端粒酶活性、下调hTERT mRNA表达有关。Objective To investigate the effects of Adriamycin （ADR） conibined with sodium Butyrate （NaBu） on HeLa cell proliferation and telomerase activity. Methods the dynamic cytotoxicity of ADR or / and NaBu was evaluated by MTT assay. The telomerase activity was measured by TRAP and silver staining. The expression of hTERT was assessed by RT-PCR. Results HeLa cell growth was inhibited by ADR or / and NaBu. IC50 of ADR alone was 6 000 nmol/L, and decreased to 320 nmol/L when conibined with NaBu. This inhibition of ADR and NaBu＋ADR were dose-dependant. The telomerase activities of Hela cells were reduced after treated 48 hours with ADR alone or with NaBu, which were dose-dependant. The difference of both treatments at the concentration of 100 nmol/L was statistically significant （P 〈 0.05）. hTERT mRNA of HeLa cells was reduced in RT-PCR,which was consistent with the telomerase activity. The difference of both treatments at the concentration of 10 nmol/L and 100 nmol/L were stistically significant （ P 〈 0.05 ）. Conclusion The application of ADR and NaBu can inhibit the proliferation of HeLa cells, and the combination of those two drugs has synergistic effect, which is related to the inhibi- tion of telomerase activity and the derease of hTERT mRNA transcription.

关 键 词：宫颈肿瘤 阿霉素 丁酸钠 端粒酶 人端粒酶逆转录酶 

分 类 号：R737.33[医药卫生—肿瘤]