手持式ATP生物荧光检测仪研制  

Development of Handheld ATP Detecting System Based on Bioluminescence

作　　者：周爱玉[1] 罗金平[1] 岳伟伟[1] 何保山[1] 杨庆德[1] 蔡新霞[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室

出　　处：《传感技术学报》2008年第4期543-546,共4页Chinese Journal of Sensors and Actuators

基　　金：北京市科学技术委员会重大项目资助(Z0006302040191);国家自然科学基金项目资助(60576049);国家高科技研究计划资助(863)项目(2007AA03Z428)

摘　　要：针对微量三磷酸腺苷（ATP）检测需求,利用生物荧光技术,设计了一种手持式ATP检测仪。该检测仪以小型光电倍增管PMT为光电转换器件,通过有效的干扰屏蔽和信号采集放大电路设计,实现了ATP反应释放微弱荧光信号的采集。以10^-15-10^-9mol/L标准ATP样品为检测对象,以大型分析仪器F4500为对比仪器,进行了梯度对比实验,结果表明,本仪器可检测最低ATP浓度为10^-14mol/L,是F4500最低检测限的1/100。检测时间为10s时,在10v-14-10^-9mol/L浓度范围内,本仪器响应值与标准ATP样品浓度在对数坐标下线性相关,相关系数达到了0.9902。A handheld Adenosine Triphosphate （ATP） detecting system based on bioluminescence was described. In the system, a small photomultiplier tube （PMT） module was used to measure the weak bioluminescence released from ATP reaction. The system was used to detect ATP in the range of 10^-15-10^-9 mol/L. The results were compared to that of fluorescence spectrophotometer F4500 which showed the lowest ATP concentration the system could detected was 10^-14mol/L which was 1/100 of that F4500 could do. In 10s＇ time, the response signals correlated well to the ATP concentration in the range of 10^-14- 10^-9 mol/L with the correlation coefficient of 0. 9902.

关 键 词：三磷酸腺苷 生物荧光 手持式检测仪 光电倍增管 

分 类 号：TN402[电子电信—微电子学与固体电子学] TE142[石油与天然气工程—油气勘探]