鸡传染性支气管炎的多重RT-PCR检测及793／B血清型的鉴定  

Detection of infectious bronchitis virus and differentiation of the 793/B serotype by multiplex RT-PCR

作　　者：孟凡磊[1] 刁有祥[1] 张伟[1] 王辉[1] 刘方娜[1] 王妮[1] 马雪杰[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东大学政治学与公共管理学院,山东济南250100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第6期45-48,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：济南市科技攻关项目(41054)

摘　　要：【目的】建立鸡传染性支气管炎病毒(IBV)快速、准确的检测方法。【方法】根据GenBank中已经发表的793/B型和多株IBV的N基因和S1基因,对比分析后以N基因的保守序列设计合成了1对引物,跨度为582bp;以793/B的S1基因设计合成了1对特异型引物,跨度为891 bp;建立了能检测出IBV并能够同时鉴定出793/B血清型的实验室一次性PCR诊断方法。【结果】多重RT-PCR检测表明,793/B血清型可出现582和891 bp 2条核酸片段,而其他血清型只出现582 bp 1条核酸片段,新城疫病毒、传染性喉气管炎、鹅副粘病毒则无任何条带出现。【结论】所建立的TR-PCR方法具有一定的特异性,可用于IBV的快速检测和793/B血清型的临床诊断。[Objective] A Multiplex RT-PCR was optimized to simultaneously detect IBV and 793/B to save detection time. [Method] Two sets of primers were designed according to the sequences of 793/B and other IBV at the Genbank. [Result] The products of 582 and 891 bp were generated only with RNA from 793/B,where as there was only 582 bp product with RNA from other IBV. However,the RT-PCR failed to detect NDV,ILTV and GPV. [Conclusion] The results showed that the established multiplex RT-PCR technique provided a more sensitive method for diagnosis and epizootic study of the IBV.

关 键 词：多重RT-PCR 传染性支气管炎 793/B血清型 检测方法 

分 类 号：S858.316.3[农业科学—临床兽医学]