蛋白质中二硫键的定位及其质谱分析  

Protein Disulfide Bond Determination and Its Analysis by Mass Spectrometry

作　　者：仇晓燕[1] 崔勐[1] 刘志强[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所长春质谱中心

出　　处：《化学进展》2008年第6期975-983,共9页Progress in Chemistry

基　　金：国家自然科学基金项目(No30672600,20675079)资助

摘　　要：二硫键是一种常见的蛋白质翻译后修饰,对稳定蛋白质的空间结构,保持及调节其生物活性有着非常重要的作用。因此,确定二硫键在蛋白质中的位置是全面了解含二硫键蛋白化学结构的重要方面。在众多实验方法中,现代质谱技术因其操作简单、快速、灵敏等优点而成为分析二硫键的重要手段。本文介绍了目前主要的定位二硫键的方法以及质谱在二硫键定位分析中的应用与进展。Disulfide bonds are one of the most common covalent posttranslational modifications of proteins. They play an important role in maintaining the three-dimensional structures of proteins and their biological activities. Therefore, the determination of disulfide bonds becomes an important aspect for obtaining a comprehensive understanding of the chemical structure of a protein. Numerous experimental methods have been developed for the determination of disulfide bonds in proteins. Modern mass spectrometry has developed as an important tool for the analysis of disulfide bond patterns due to its advantages of being simple, rapid and sensitive. Some useful methods for assignment of disulfide bonds in proteins are introduced, the developments and applications of mass spectrometry in this area are reviewed.

关 键 词：二硫键定位 质谱 串联质谱 三羧乙基膦 稳定同位素标记 

分 类 号：O657.63[理学—分析化学] Q51[理学—化学]