代谢木糖重组酿酒酵母的初步构建  

Initial construction of recombinant Saccharomyces cerevisiae for metabolizing Xylose

作　　者：张金鑫[1] 张亚珍[1] 杨忞[1] 李颖[1] 高文[1] 杨秀山[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《可再生能源》2008年第2期35-39,共5页Renewable Energy Resources

基　　金：国家"863"计划资助项目(2002AA514010;2001AA514024)

摘　　要：为使酿酒酵母(Saccharomyces cerevisiae)代谢木糖产乙醇,须要在酿酒酵母胞内表达木糖代谢途径的关键酶基因——木糖还原酶基因xyl1和木糖醇脱氢酶基因xyl2,这2种酶基因的相对表达水平对乙醇的产生和木糖醇的积累具有重要影响。该研究初步构建了含有诱导型启动子控制的木糖还原酶基因xyl1的酵母表达载体pYes2-xyl1和含有组成型强启动子控制的木糖醇脱氢酶基因xyl2的酵母表达载体pDR195-xyl2,同时,在国内首次克隆出热带假丝酵母(Candida tropicalis)的木糖醇脱氢酶基因。这2个表达载体的构建为后续重组酵母的构建提供了重要基础。In order to enable Saccharomyces cerevisiae to utilize and metabolize xylose to produce ethanol, it is necessary to express the key enzymes of xylose metabolism in the cell of Saccha- romyces cerevisiae.The gene codes of xyl1 and xyl2 are for the two key enzymes which are xylose reductase and xylitol dehydrogenase respectively. The relative expression level of these two enzymes has a great influence on the accumulation of xylitol and the yield of ethanol. This research has constructed an yeast expression vector pYes2-xyl1 containing xyl1 under the control of an inducible promoter and another expression vector pDR195-xy12 containing xyl2 under the control of a constitutive strong promoter, and has cloned xyl1 from Candida tropicalis for the first time nationwide. The construction of these two vectors provides an important basis for the further establishment of recombinant Saccharomyces cerevisiae.

关 键 词：重组酿酒酵母 木糖 乙醇 木糖还原酶基因 木糖醇脱氢酶基因 

分 类 号：Q291[生物学—细胞生物学] Q933