不同冷冻温度下保存大鼠坐骨神经的实验研究  

REGENERATION ACROSS COLD PRESERVED PERIPHERAL NERVE ALLOGRAFTS STORED IN THE GLYCEROL OF DIFFERERT temparature

作　　者：邹永根[1] 伍光辉[1] 彭忠毅[1] 

机构地区：[1]泸州医学院附属中医院骨科,646000

出　　处：《内蒙古中医药》2008年第5期42-44,共3页Inner Mongolia Journal of Traditional Chinese Medicine

摘　　要：目的:观察不同冷冻温度下保存的异体神经对周围神经缺损修复的效果。方法:取96只同种属雄性大鼠,其中24只为取材组,72只随机分为对照组(A、B组)和实验组(C、D、E、F组),每组12只。A组为新鲜自体神经移植组,B组为新鲜异体神经移植组,C、D、E和F组分别为经4℃、-50℃、-80℃和-196℃甘油保存3周的神经移植组。术后2周,进行免疫组织化学观察;术后9、16周进行大体观察、光镜检察、电生理测定。结果:术后2周,B组移植段见明显T淋巴细胞浸润,C、D、E组次之,A组及F组未见T淋巴细胞。术后9周及16周,从神经再生效果评价看:A组神经再生效果最好,F组次之,C、D、E组再次,B组最差。结论:在各冷冻组中,以-196℃组神经再生效果最好,接近新鲜自体神经移植组。Objective： To investigate the regeneration of peripheral nerve allografts stored in the glycerol of different temperature.Methods： 96 male wistar rats, were randomly divided into 7 groups. Group A and B were the control groups, each included 12 rats; all underwent fresh femoral nerve autcgraft and allograft transplantation respectively. 24 rats in group G were sacrificed for the 48 femoral nerves, which were randomly divided into 4 groups, 12 nerves in each group, and were stored in glycerol for 3 weeks at 4℃, - 50℃, - 80℃ and - 196℃ respectively. After 3 weeks storage, 4 × 12 nerves in each temperature group were randomly transplanted into 4 × 12 rats in target rats group C, D, E and F respectively. 2 weeks after transplantation, two grafts of transplanted nerves in group A-- F were harvested randomly and examined with immunohistochenaistry to observe the infiltration of lymphocyte to the grafts and regeneration of sciatic nerve and fiber. 9 and 16 weeks after transplantation, the regeneration of sciatic nerve was examined with histology, electro- physiology and electron microscope.Results： Wallerian degeneration was observed in the proximal and distal ends of all grafts of nerves harvested at 2 weeks post- engraftment; infiltration of T lymphocytes at the grafts in group B was significant, less T lymphocytes were seen in group C, D and E, while no T lymphocytes were seen in group A and F. Collagenoblast proliferation was observed in group B, Schwarm cell proliferation in group A and F, while both collagenoblast and Schwarm cell proliferation were observed in group C, D and E. 9 and 16 weeks post- engraftment, grafts in group B were severely adhered to the near tissue, adherence in group A and F were light, while group C, D and E came between. Nerve regeneration of group A was the best, and group F came second, the next were group C, D and E, while group B was the worst.Conclusion： The best storage temparature is - 196℃.

关 键 词：周围神经 神经移植 预处理 温度 

分 类 号：R-332[医药卫生]