血管生成素-2／受体-2在肝癌血管新生中的作用  

Role of Angiopoietin-2 and Tie-2 in the Angiogenesis of Primary Hepatic Carcinoma

作　　者：焦辉[1] 郭春良[2] 王宏[2] 

机构地区：[1]武警8664部队医院 [2]武警医学院附属医院普外科,天津市300162

出　　处：《中国肿瘤临床》2008年第11期644-647,共4页Chinese Journal of Clinical Oncology

基　　金：天津市应用基础研究计划面上基金资助(编号:05YFJMJC08100)

摘　　要：目的:分析Ang-2、Tie-2和CD34在原发性肝癌(primary hepatic carcinoma,PHC)组织、癌旁组织、肝硬化组织及正常肝组织中的表达特征,研究Ang-2和Tie-2与原发性肝癌血管新生的相关性,探讨Ang-2和Tie-2在原发性肝癌血管新生中的作用,以及与临床病理之间的关系。方法:采用免疫组织化学SP法检测32例原发性肝癌癌组织及癌旁组织中Ang-2、Tie-2和CD34的表达,以15例肝硬化和10例正常肝组织中Ang-2、Tie-2和CD34的表达水平作为对照,对其表达差异进行统计学分析。结果:Ang-2、Tie-2和CD34在原发性肝癌组织中表达明显上调,在肝硬化组织的表达较弱,在正常肝组织不表达。肝癌与癌旁组织Ang-2/Tie-2的表达差异有显著性。肝癌、肝硬化组织中Ang-2/Tie-2的表达差异有显著性。在PHC组织中Ang-2/Tie-2的表达与MVD呈正相关性。Ang-2和Tie-2与MVD、肝癌分化程度以及侵袭性高低密切相关。结论:PHC组织Ang-2和Tie-2的表达与PHC血管新生及MVD有显著相关性。Ang-2通过与Tie-2特异性结合发挥生物效应。Ang-2和Tie-2在原发性肝癌血管新生中起重要作用。Objective： To investigate the expression of Angiopoietin-2 （Ang-2）, Tie-2 and CD34 in hepatoma tissues,para-cancer tissues, tissues of hepatic cirrhosis and normal tissues, to study the correlation of Ang-2 and tyrosine kinase receptor Tie-2 with the angiogenesis in primary hepatic carcinoma （PHC）, and to explore the role of Ang-2 and Tie-2 in the angiogenesis of PHC. Methods： Specimens from 32 PHC patients and 15 patients with hepatic cirrhosis and 10 samples of normal liver tissue were detected with S-P immunohistochemistry. Results： Ang-2, Tie-2 and CD34 were significantly up-regulated in tissues of PHC compared with tissues of hepatic cirrhosis and normal liver tissues. The expression of Ang-2, Tie-2 and CD34 was absent in normal liver tissues. The expression of Ang-2, Tie-2 and CD34 in tissues of liver carcinoma was higher than in tumor adjacent tissues. The expression of Ang-2, Tie-2 and CD34 in tissues of liver carcinoma was significantly higher than in hepatic cirrhosis and normal liver. There was a positive correlation between Ang-2/Fie-2 and MVD. The expression of Ang-2 and Tie-2 in tissues of PHC was correlated with MVD, histopathological grading and invasion of PHC. Conclusion： The expression of Ang-2 and Tie-2 in primary hepatic carcinoma was correlated with angiogenesis and MVD of hepatic carcinoma. Ang-2 can specifically bind with Tie-2. Ang-2 and Tie-2 may play a critical role in promoting tumor angiogenesis of PHC.

关 键 词：原发性肝癌 血管新生 血管生成素-2 血管生成素-2受体 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤] R541.4[医药卫生—临床医学]