沉默hHBrk1基因对肺癌细胞增殖及迁移能力的影响  

Effect of silencing hHBrk1 on proliferation and migration of lung carcinoma cells

作　　者：虞红珍[1] 祝敏[1] 周平坤[2] 隋建丽[2] 王豫[2] 徐勤枝[2] 汪思应[1] 

机构地区：[1]安徽医科大学基础医学院病理学与病理生理学教研室,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国病理生理杂志》2008年第6期1106-1110,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30570550);北京市自然科学基金资助项目(No5042023;No7062052);安徽省人才开发基金资助项目(No2002Z035);安徽省2006研究实验基地科研带头人培养专项基金资助

摘　　要：目的:研究玉米Brick1的人类同源基因hHBrk1(human homology of Brick1)对肿瘤细胞增殖及迁移能力的影响。方法:用质粒介导的siRNA(small interfering RNA)技术建立hHBrk1基因表达沉默的肺癌细胞模型;用细胞生长曲线、克隆形成率实验及流式细胞术分别比较不同处理的95D细胞增殖及细胞周期的变化;用Tran-swell细胞侵袭实验系统研究hHBrk1表达沉默对肺癌细胞迁移能力的影响。结果:成功筛选出hHBrk1基因表达抑制的细胞模型;hHBrk1表达沉默细胞增殖能力和细胞周期与对照组相比无明显改变,但hHBrk1表达沉默细胞迁移能力明显减弱。结论:hHBrk1可能参与调控肺癌细胞迁移能力。AIM： To observe the effect of hHBrkl gene on proliferation and migration of lung carcinoma cells. METHODS： Recombinant plasmids harboring 19 -nt- ong small interfering RNA （siRNA） were constructed and tested to selectively downregulate hHBrkl gene in human lung cancer 95D cell line in vitro by stable transfection with Lipo-fectamine 2000. The mRNA level of the ceils transfected with siRNA plasmids were monitored by Northern blotting and RT-PCR. Growth curve and flow cytometry were applied to determine the cell proliferation and cell cycle. Ability of cell mi- gration was measured by Trans -well system. RESULTS： hHBrkl gene was silenced by targeting siRNA, and stable silen-cing cell model was constructed. No difference in proliferation and clone formation between hHBrkl silencing cells and control cells was observed. The ability of migration was decreased in hHBrkl silencing cells as compared with control cells. CONCLUSION ： hHBrkl may play an important role in migration of the lung cancer cells.

关 键 词：基因 hHBrkl RNA干扰 肺肿瘤 细胞运动 细胞增殖 基因沉默 

分 类 号：R363[医药卫生—病理学]