孕酮对人树突状细胞成熟和免疫功能的影响  

Effects of progesterone on the maturation and immunologic function of dendritic cells from human peripheral blood

作　　者：沈媛[1] 曾耀英[2] 肇静娴[2] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632 [2]暨南大学组织移植与免疫实验室,广东广州510632

出　　处：《中国病理生理杂志》2008年第6期1143-1147,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划项目“973”资助(NoG1999054303);国家自然科学基金重点项目资助(No30230350);广东省“十五”重大科技专项(NoA302020204);

摘　　要：目的:研究孕酮(P4)对人外周血来源树突状细胞(DCs)成熟和免疫学功能的影响。方法:在人外周血来源DCs体外培养时加入两种浓度的P4(10-7mol/L和10-6mol/L)处理,光镜和电镜下观察DCs的生成情况及形态变化,以流式细胞仪分析各组细胞的免疫表型,用ELISA方法测定其分泌的IL-10和IL-12水平,[3H]-TdR掺入法检测体外混合淋巴细胞反应中DCs刺激同种反应性T细胞的增殖能力。结果:加入P4培养后,DCs树突状和膜面状伪足减少,表达低水平MHC-II分子和共刺激分子CD40、CD80和CD86,其分泌的IL-10水平升高,IL-12水平明显下降,DCs刺激同种反应性T细胞的功能显著下降。结论:P4抑制人外周血来源DCs的成熟,对其免疫学功能具有负性调节作用。AIM ： To study the effects of progesterone （ P4 ） on the maturation and immunologic function of dendritic cells （DCs） from human peripheral blood. METHODS： Cultured DCs were treated with P4 at doses of 10^-7 mol/L and 10^-6 mol/L. The morphologic changes were observed under the scanning electronic microscope. The immuno-phenotypes of DCs in control and treated groups were analyzed by flow cytometry. IL - 10 and IL - 12 production in culture supernatant was examined by ELISA assay. The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of [ ^3 H] -TdR. RESULTS： Compared with control group, cultured DCs in the presence of P4 displayed less dendritic pseudopod, expressed low levels of MHC - 11, CD40, CD80 and CD86, and exhibited weakly activity in stimulating the proliferation of allogeneic T cells. Increase in IL - 10 production and decrease in IL - 12 production were observed. CONCLUSION： P4 exerts negative effect on the maturation and immunologic function in dendritic cells from human peripheral blood.

关 键 词：树突细胞 孕酮 免疫功能 

分 类 号：R363[医药卫生—病理学]