Hsa-miR-93在肝癌中的作用机制研究  

The roles of hsa-miR-93 on the hepatocellular carcinoma

作　　者：李文晰[1] 梁琳慧[1] 何祥火[1] 万大方[1] 顾健人[1] 

机构地区：[1]上海交通大学上海市肿瘤研究所癌基因及相关基因国家重点实验室,上海200032

出　　处：《胃肠病学和肝病学杂志》2008年第6期478-480,484,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的观察hsa-miR-93对肝癌细胞生长和细胞周期的影响,以分析其在肝癌中的作用机制。方法用real time RT-PCR法分析hsa-miR-93在人肝癌和癌旁肝组织的表达情况。此后构建hsa-miR-93过表达载体,同时合成二甲氧修饰的hsa-miR-93的反义核苷酸序列,二者转染肝癌细胞株观察它们对肝癌细胞生长及细胞周期的影响,分别以空载体和无关寡核苷酸序列为对照。转染后CCK-8法检测肝癌细胞生长情况,通过流式细胞仪分析细胞周期。结果hsa-miR-93在肝癌组织中表达明显高于癌旁肝组织。hsa-miR-93过表达载体可以促进肝癌细胞生长,促进细胞周期的G1/S期转换,而hsa-miR-93反义序列抑制肝癌细胞生长,使肝癌细胞阻滞于G1期(P<0.05)。结论hsa-miR-93通过促进细胞周期的G1/S期转换而促进肝癌细胞生长,提示hsa-miR-93可能是肝癌的发病机制中一个重要分子。Objective To investigate the function of hsa-miR-93 in hepatocellular carcinoma by analyzing the effect of hsa-miR-93 on the growth and cell cycle of liver cancer cells. Methods The expression of hsa-miR-93 in hepatocel- lular carcinoma and corresponding non-cancerous tissues were analyzed by real time RT-PCR. Then we investigated the effect of hsa-miR-93 on cell growth and cell cycle by transfection the overexpression vector or antisense sequence of hsa- miR-93 into liver cancer cells. Cell growth was studied with CCK-8 assay and cell cycle was analyzed by FACS. Results The expression of hsa-miR-93 was significantly higher in hepatocellular carcinoma tissue than in corresponding non- cancerous tissues. The hsa-miR-93 overexpression vector could promote the growth of liver cancer cells by regulating G1/ S transition. The antisense sequence of hsa-miR-93 could suppress the growth of liver cancer cells by introduction of cell cycle arrest in G1. Conclusion Hsa-miR-93 may be an important molecule in HCC, which promote the cell growth by regulating G1/S transition.

关 键 词：hsa-miR-93 肝癌 发病机制 

分 类 号：R735.7[医药卫生—肿瘤]