系统性红斑狼疮患者骨髓间质干细胞超微结构及细胞骨架的特征  

Characterization of the ultrastructure and cytoskeleton of bone marrow- derived mesenchymal stem cells in patients with systemic lupus erythematosus

作　　者：顾志峰[1] 赵盛楠[2] 张华勇[2] 徐婷[2] 金鸥阳[2] 周康兴[2] 刘海鸥[3] 张天一[3] 孙凌云[2] 

机构地区：[1]南京中医药大学,210046 [2]南京大学医学院附属鼓楼医院风湿免疫科 [3]南通大学医学院电镜室

出　　处：《中华风湿病学杂志》2008年第6期397-400,I0003,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目（30772014）;教育部高校博士点专项基金项目（20050315001）;江苏省科教兴卫工程重点人才基金资助项目（苏卫科教[2007]2号）;南京市科技发展项目基金（200702068）

摘　　要：目的 探讨系统性红斑狼疮（SLE）患者骨髓间质干细胞（MSCs）的超微结构及细胞骨架的形态学特征。方法 体外培养SLE患者的MSCs后，透射电子显微镜（TEM）、激光共聚焦显微镜（LCM）观察超微结构及肌动蛋白、纽蛋白的表达。结果 SLEMSCs透射电子显微镜下多数细胞内呈衰老征象，可见大量自噬体；细胞边缘应力纤维增多，排列紊乱，组成粗大的强荧光团，核周围应力纤维减少；纽蛋白在细胞边缘排列紊乱，细胞核周围增多。结论 SLEMSCs超微结构及细胞骨架的改变是细胞扩增不良的原因之一。Objective To explore ultrastructure and cytoskeleton characteristics of bone marrowderived mesenchymal stem cells （MSCs） in patients with systemic lupus erythematosus （SLE）. Methods MSCs were isolated from bone marrow of 2 SLE patients and 2 healthy controls. Their ultrastructures were examined by transmission electron microscope （TEM）. The expression pattern of actin and vinculin was assessed by laser confocal microscopy （LCM）. Results MSCs in patients with SLE presented with signs of ageing and lots of autophagosome could be found in most of the cells.F-actin was aggregated and condensed at the border of cytoplasm. Vinculin was arranged disorderly and condensed in the cytoplasm. Conclusion The change of uhrastructure and cytoskeleton patterns of bone marrow derived mesenchymal cells of SLE patients may play an important role in the abnormal proliferation of these cells in vitro.

关 键 词：红斑狼疮 系统性 骨髓间质干细胞 超微结构 肌动蛋白 纽蛋白 

分 类 号：R593.241[医药卫生—内科学]