ALCL染色体移位的间变性淋巴瘤激酶的表达及其与预后的关系  

Expression of ALK Protein in Large Cell Lymphoma with ALCL Chromosome Translocation in Relation to Prognosis

作　　者：季天海[1] 李慧灵[1] 蒋会勇[1] 赵彤[1] 余英豪[2] 

机构地区：[1]南方医科大学附属南方医院病理科,广东广州510515 [2]南京军区福州总医院病理科,福建福州350025

出　　处：《中国实验血液学杂志》2008年第3期543-546,共4页Journal of Experimental Hematology

基　　金：广东省社会发展攻关基金项目资助(编号B30301);广州市科技计划基金项目资助(编号2002Z34061)

摘　　要：本研究应用组织芯片技术探讨间变性大细胞淋巴瘤(anaplastic large cell lymphoma,ALCL)中染色体移位产生的间变性淋巴瘤激酶(ALK)蛋白的表达及其与患者年龄和预后的关系。建立包含30例ALCL及2例对照组织共96点阵的组织芯片,采用免疫组织化学SP法检测ALK蛋白表达,并应用SPSS软件进行统计学分析。结果显示:①ALK蛋白在2例皮肤原发ALCL患者中表达均为阴性,28例系统性ALCL中有20例(71.4%)表达为阳性,其表现主要为细胞浆和(或)细胞核棕黄色;②ALK蛋白阳性组的年龄低于ALK蛋白阴性组,两者统计学差异具有显著性(p<0.05);③ALK蛋白阳性组的预后好于ALK蛋白阴性组,两者统计学差异具有显著性(p<0.05)。结论:ALK蛋白的表达在ALCL中有很高的发生率,尤其是在低年龄组的患者,它可以作为ALCL的诊断、鉴别诊断和判断预后的一个独立重要指标。The aim of this study was to investigate the expression of anaplastic lymphoma kinase （ALK） protein resulted from chromosome translocation in anaplastic large cell lymphoma （ALCL） and its relationship with the age and prognosis of patients with ALCL. The tissue microarray including 30 cases of ALCL and 2 normal control tissues were estatlished, the expression of anaplastic lymphoma kinase （ALK） protein was detected by immunohistochemistry, the statistical analysis of detected results was carried out by SPSS software. The results showed that the ALK protein was expressed negatively in 2 cases of primary skin ALCL, but in 20 out of 28 cases of systematic ALCL the ALK protein was expressed positively and mainly located in cytoplasm and/or nucleus （71.4%）. Clinically, the patients with ALK expression were younger than those without ALK expression （p 〈 0.05 ）. The prognosis of patients with ALK expression was better than those without ALK expression （p 〈 0.05 ）. It is concluded that there is a high incidence of ALK expression in ALCL, especially in younger group. ALK expression may be an useful and independent marker for the differential diagnosis and prognosis evaluation of ALCL.

关 键 词：间变性淋巴瘤 间变性淋巴瘤激酶 染色体 组织芯片 

分 类 号：R733.1[医药卫生—肿瘤]