rhG-CSF动员的外周血采集物与稳态骨髓中CD4^＋CD8^＋初始及记忆T细胞亚群的比较  

Comparative Analysis of Naive and Memory CD4^+ and CD8^+ T-cell Subsets in rhG-CSF Mobilized Peripheral Blood and Steady-state Bone Marrow

作　　者：霍明瑞[1] 赵翔宇[1] 常英军[1] 罗小华[1] 黄晓军[1] 

机构地区：[1]北京大学人民医院,北京大学血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2008年第3期605-609,共5页Journal of Experimental Hematology

基　　金：教育部新世纪优秀人才基金(编号NCET-04-0011);首都医学发展科研基金(编号2005-1010);"863"计划(编号2006AA02Z4A0)

摘　　要：本研究探讨重组人粒细胞集落刺激因子(rhG-CSF)动员的外周血采集物(G-PB)与稳态骨髓(SS-BM)中CD4+、CD8+初始、记忆T细胞亚群的差异。依据细胞表面CD45RA和CD62L的表达将CD4+、CD8+T细胞划分为初始T细胞(naive,CD45RA+CD62L+)、中心记忆T细胞(central memory,TCM,CD45RA-CD62L+)、效应记忆T细胞(effector memory,TEM,CD45RA-CD62L-)、终末分化效应记忆T细胞(terminally differentiated effector memory,TTD,CD45RA+CD62L-)。用流式细胞仪测定G-PB与SS-BM中CD4+、CD8+初始和记忆T细胞的比例组成,并计算每微升采集物中各细胞亚群的绝对数量。结果表明:G-PB中CD4+、CD8+T细胞的比例组成及CD4+/CD8+比值均高于SS-BM(p<0.05);G-PB中CD4+初始T细胞的比例显著低于SS-BM(p<0.001),而CD4+TEM比例显著高于SS-BM(p<0.001);G-PB中CD8+初始和记忆T细胞亚群的比例与SS-BM无显著差异(p>0.05);与SS-BM相比,G-PB中CD4+CD62L+T细胞的比例明显降低(p=0.001),每微升G-PB移植物中的各淋巴细胞亚群均明显高于SS-BM(p<0.001)。结论:G-PB与SS-BM中CD4+、CD8+初始和记忆T细胞亚群在相对和绝对数量方面存在明显的差异,这种差异可能影响G-PB和SS-BM移植后急性、慢性移植物抗宿主病发生率及其程度的异同。The study was aimed to analyze the difference of naive and memory CD4 ^＋ and CD8^＋T-cell subsets between steady-state bone marrow （SS-BM） and recombinant human granulocyte colony-stimulating factor （rhG-CSF） mobilized peripheral blood grafts （G-PB）, Four CD4^＋ and CD8 ^＋ T-cell subsets classified according to the expression of CD45RA and CD62L were determined by three-color flow cytometry. The results showed that the percentage of CD4 ^＋ , CD8 ^＋ T-cell subsets and the ratios of CD4/CD8 in G-PB were significantly higher than those in SS-BM （p 〈 0.05 ）. The percentage of CD4^＋ naive T-cells in G-PB was significantly lower than that in SS-BM （p 〈0.001 ）. As compared with SS-BM, the percentage of CD4 ^＋ effector memory T-cells was significantly high in G-PB （p 〈0.001 ）. There were no significant differences in the percentages of the four CD8 ^＋ T-cell subsets between SS-BM and G-PB （p 〉 0.05）. The percentage of CD4 ^＋ CD62L^＋ T-cells in G-PB was significantly lower than that in SS-BM （p = 0. 001 ）. The absolute numbers of CD4 ^＋ and CD8 ^＋ T-cell subsets, the eight naive and memory CD4 ^＋ and CD8 ^＋ T-cell subsets were signifi- cantly higher in G-PB than those in SS-BM （p 〈0.001 ）. It is concluded that the difference of naive and memory CD4 ^＋ and CD8 ^＋ subsets between G-PB and SS-BM may partially explain why the incidence and severity of acute graft-versushost disease （GVHD） was similar and the incidence of chronic GVHD was different after transplantation with SS-BM or G-PB.

关 键 词：骨髓移植物 外周血移植物 CD4^+T细胞 CD8^+T细胞 初始T细胞 记忆T细胞 粒细胞集落刺激因子 

分 类 号：R457.7[医药卫生—治疗学] R392.4[医药卫生—临床医学]