COX-2和VEGF与肝癌多细胞球体群集耐药性的关系  

Relationship between COX-2 and VEGF expressions and multicellular resistance in hepatocellular carcinoma multicellular spheroids

作　　者：姚煜[1] 田涛[1] 崔洁[1] 赵娜[1] 郭亚焕[1] 南克俊[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科,陕西西安710061

出　　处：《第四军医大学学报》2008年第11期991-993,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨COX-2和VEGF在肝癌多细胞球体(MCS)中的表达与肝癌群集耐药的关系.方法:以liquidoverlay技术获得肝癌HepG2多细胞球体为模型,采用透射电镜和扫描电镜比较单层细胞与MCS的形态学差异;采用MTT法和流式细胞技术比较ADM和5-FU对单层细胞和MCS的生长抑制率和细胞凋亡率;采用免疫组化和图像分析技术比较COX-2和VEGF在单层细胞和多细胞球体表达的差异.结果:与单层细胞相比,多细胞球体细胞间黏附广泛而紧密,可见中间连接和桥粒连接.不同浓度的ADM和5-FU作用48h,MCS细胞的生长抑制率和细胞凋亡率均低于单层细胞,差异有统计学意义(P<0.05).免疫组化结果显示,COX-2和VEGF在MCS上高表达(P<0.05).结论:肝癌MCS具有群集耐药性,可能与其高表达COX-2和VEGF有关.AIM： To examine the expressions of COX-2 and VEGF in hepatocellular carcinoma muhicellular spheroids （MCS） and their relationship with multicellular resistance （MCR）. METHODS： Hepatocellular carcinoma HepG2 MCS were obtained by liquid overlay technique and observed by transmission electron microscope and scanning electron microscope. After treatment with ADM and 5-FU for 48 h, cell growth inhibition rate and cell apoptosis rate of monolayer cells and MCS were analyzed by MTr assay and flow cytometry. The expressions of COX-2 and VEGF in monolayer cells and MCS were examined by immunohistochemical method and image analysis. RESULTS： Compared with monolayer cells, MCS had more extensive and compact cell adhesion and intermediate junction and desmosome were found within them. Treated with ADM and 5-FU for 48 h, cell growth inhibition rate and cell apoptosis rate were significantly lower in MCS than in monolayer cells （ P 〈 0. 05 ）. The immunohistochemical results showed COX-2 and VEGF expressions were higher in MCS than in monolayer cells （ P 〈 0.05 ）. CONCLU- SION ： Hepatoeellular carcinoma MCS has muhicellular resistance and the possible mechanisms may be associated with the upregulation of COX-2 and VEGF

关 键 词：肝癌 群集耐药 环氧化酶-2 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]