大蒜新素对大鼠变应性鼻炎模型血浆中白细胞介素4和γ干扰素的影响  

Effect of garlicin on the serum levels of interleukin 4 and interferon γ in allergic rhinitis model in rats

作　　者：李玉晓[1] 陈冬[1] 李添应[1] 冯炼强[2] 许庚[1] 文卫平[1] 

机构地区：[1]中山大学附属第一医院耳鼻咽喉科医院李,广州510080 [2]中山大学基础医学院免疫学教研室

出　　处：《中华耳鼻咽喉头颈外科杂志》2008年第6期447-450,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：广东省卫生厅基金（A2005214）

摘　　要：目的探讨大蒜新素对大鼠变应性鼻炎模型外周血中γ干扰素（INF-γ）和白细胞介素4（IL-4）的影响。方法健康雌性SD大鼠30只，随机分为对照组、空白组和实验组，每组10只。实验组用卵清蛋白辅以氢氧化铝和灭活百日咳杆菌佐剂建立变应性鼻炎模型，成功后连续腹腔注射大蒜新素10d；对照组造模方法同实验组，成功后腹腔注射等体积的生理盐水；空白组除无卵清蛋白致敏和激发外，方法同对照组。颈内静脉取血，分离血浆，采用酶联免疫吸附试验（ELISA）检测血浆中IL-4和INF-γ的含量。结果实验组、对照组和空白组大鼠外周血浆中IL-4水平（-↑x±s，下同）分别为（22．81±8．79）pg／L、（41．43±4．93）pg／L和（9．93±2．07）pg／L；INF-γ的水平分别是（22．32±11．20）pg／L，（11．35±2．45）pg／L和（21．69±5．93）pg／L。实验组血浆中IL-4的水平低于对照组而高于空白组，差异具有统计学意义（t值分别为3．22及4．17，P值均〈0．05）；实验组血浆中INF-γ水平高于对照组（t=3．84，P〈0．05），而与空白组相比差异无统计学意义（t=1．47，P〉0．05）。结论大蒜新素能降低大鼠变应性鼻炎模型外周血中IL-4的水平，同时提高INF-γ的水平，对Th1和Th2细胞因子水平具有调节作用。Objective To investigate the effect of garlicin on the levels of interferon γ （INF-γ） and interleukin 4 （IL-4） in blood of allergic rhinitis rat model. Methods Thirty healthy female SD rats were randomly divided into 3 groups： control group, negative control group and experimental group, 10 rats for each group. Ten rats （experimental group ） were sensitized and intranasally challenged by ovalbumin, aluminium hydroxide hydrate gel and Bordetella pertussis inactive microorganism suspension adjuvants, as allergic rhinitis models, and then injection of garlicin（0.4 ml） intraperitoneally per day for 10 days. Control group rats were immunized as experimental group, and then injection of physiological saline as equal volume as garlicin. Negative control group rats were investigated using physiological saline. Blood of intrajugnlar vein of rat was extracted for separated plasma. Enzyme liked immunosorbent assay （ELISA） was utilized to detect the serum levels of IL-4 and IFN-γ. Results The serum levels （ -↑x ± s） of IL-4 were （22. 81 ± 8.79） pg/L, （41.43±4.93）pg/L, （9.93 ±2.07）pg/L, and those of IFN-γ were（22.32 ±11.20） pg/L, （ 11.35 ± 2.45 ）pg/L and （21.69 ± 5.93 ）pg/L, respectively, among experimental group, control group and negative control group. The serum level of IL-4 in experimental group rats was lower than value of control group rats （t =3.22,P 〈0. 05）, while higher than negative control group（t =4. 17,P 〈0.05）. The serum level of IFN-γ was increased significantly in experimental group rats with significant difference when compared with value of control group rats （ t = 3.84, P 〈 0.05 ）, while no difference was shown between experimental group and negative control group（ t = 1.47, P 〉 0. 05 ）. Conclusions Garlicin could increase serum level of INF-γ and decrease serum level of IL-4 significantly in allergic rhinitis rat model. It played an important role on regulating serum levels of cytokines of Th1 and Th2.

关 键 词：模型 动物 鼻炎 变应性 季节性 大蒜辣素 白细胞介素4 干扰素Ⅱ型 

分 类 号：R686[医药卫生—骨科学]