应用FACS检测CIK的抗肿瘤活性  

Detection of CIK Anti-Tumor Activity via Application of FACS

作　　者：蒋东霞[1] 赵婷茹[2] 杨晓博[1] 胡杰英[1] 徐虹[1] 宋永平[1] 

机构地区：[1]河南省肿瘤研究所,郑州市450008 [2]河南省肿瘤医院,郑州市450008

出　　处：《医药论坛杂志》2008年第11期1-2,5,共3页Journal of Medical Forum

基　　金：河南省科委专项基金资助项目(编号:341700900)

摘　　要：目的探讨体外CIK细胞(cytokine-induced killer)对肿瘤细胞的细胞毒作用。方法从健康人外周血分离淋巴细胞,经过IFNγ、CD3McAb、IL-2诱导、培养,获得CIK细胞。FACS检测CIK细胞的表型;用CFSE标记靶细胞以区分效应细胞,再以PI染色,FACS检测靶细胞的死亡率。结果CIK细胞高度表达CD3+、CD8+、CD3+CD8+、CD3+CD56+,依次为89.33%、46.26%、58.98%、30.83%。CIK对NK敏感的K562及不敏感HL60、Hela均表现出较强杀伤活性。结论细胞因子IFNγ、CD3McAb、IL-2体外诱导的单核细胞具有强大的增殖力和杀伤活性及非MHC限制性。Objective To probe into the cytotoxicity of CIK cells against tumor cells in vitro. Methods Lymphocytes cells were isolated freshly from peripheral blood of health donors by Ficoll - Hypaque density centrifugation,and the cells obtstained were induced by IFNγ, IL- 2 and CD3mAb. Phenotypes of CIK cells were analysed by FACS. Target cells were differentiated from effect cells by CFSE dying. The mortality of Target cells were determined by FACS. Results The phenotypes of CD3 ^＋ was expressed up to 89.33% , while the percentage of CD8 ^＋ was 46.26% ; with CD3 ^＋ CD8 ^＋ , it was 58.98% and went to 30. 83% when dealing with CD3 ^＋ CD56 ＋. CIK cells possess the cytotoxicity against tumor cells of NK sensitive K562, insensitive HL -60, Hela. Conclusion IFN γ, CD3McAb ,IL- 2 could induced strong peripheral lymphocytes to produced CIK cells which exert highly efficient cytotoxic effects and none - MHC limitation on tumor cells.

关 键 词：CIK细胞 细胞毒性 流式细胞术 

分 类 号：R730.45[医药卫生—肿瘤]