ET-1mRNA在弥漫性脑损伤中的表达及与脑水肿的关系  

Expression of ET-1mRNA and Edema of Brain after Diffuse Brain Injury

作　　者：李玉[1] 

机构地区：[1]河南省人民医院神经外科,郑州市450003

出　　处：《医药论坛杂志》2008年第11期3-5,共3页Journal of Medical Forum

摘　　要：目的探讨弥漫性脑损伤脑组织不同时间段ET-1mRNA表达及与脑组织水肿的关系。方法依据Marmarou's弥漫性脑损伤动物模型改进,应用SD雄性大鼠55只,随机分为2组:假手术(对照组)(n=5)和弥漫性脑损伤组(n=50),损伤组再按照不同时间段分组(n=5),损伤后大鼠自由进食饮水,按0.5h、1h、3h、6h、12h、24h、48h、72h、1周、2周等时间段处死大鼠,提取大鼠皮层脑组织。应用分析天平测定组织干重,应用脑组织干湿重比表示脑组织含水量。结果对照组,0.5h、1h、3h、6h、12h、24h、48h、72h、1周、2周时间段外伤组ET-1mRNACt值不同时间组之间差别有统计学意义;脑组织含水量对照组不同时间组之间含水量差别有统计学意义(P<0.01)。结论①弥漫性脑损伤后ET-1mRNA表达短期增加,6h就达到高峰。②弥漫性脑损伤后脑组织含水量增加,6h增幅快,12h到达较高水平,持续2周。③ET-1mRNA早期高表达可能是导致脑组织含水量增加的一个原因。Objective To determine and evaluate the transformation of the brain edema and to determine the expression of ET -1mRNA in brain following diffuse brain injury. Methods fifty -five male SD rats were randomly divided into two groups ：control （ n =5）,DBI （ n = 50 ;they were divided into ten groups again, n = 5）. Diffuse contusion and laceration of brain was produced by Marmarou＇s brain injury model. Determine and evaluate the water contained in brain and the expression of ET - 1mRNA at different time after brain injury. Results The expression of ET - 1mRNA upregulate rapidly after brain injury, it reach top at 6 hours ,then decline slowly. The expression of ET - 1mRNA are different among groups by karskal-wallis test, P 〈0. 01. The water contained in damaged brain increased rapidly at 6 hours. The water contained in damaged brain is different among groups by kurskal -wallis test. It can reach higher level at 12 ①the expression of ET - 1mRNA following diffuse brain injury is upregulated rapidly in short time. It reaches the top at 6 hours. ②The water contained in damaged brain can increase rapidly. It can reach higher level at 12 hours. ③The expression of ET - 1mRNA following diffuse brain injury can upregulate the water contained in damaged brain.

关 键 词：弥漫性脑损伤 血管内皮素-1 脑水肿 

分 类 号：R-332[医药卫生]