离体条件下西黄松成熟合子胚不定芽的诱导及植株再生(英文)  

Adventitious Bud Induction and Plantlet Regeneration in vitro from Mature Zygotic Embryos of Ponderosa Pine

作　　者：李科友[1] 唐德瑞[1] 李林[1] 朱海兰[1] 赵忠[1] 侯琳[1] 

机构地区：[1]西北农林科技大学林学院,杨凌712100

出　　处：《林业科学》2008年第5期38-45,I0001,共9页Scientia Silvae Sinicae

基　　金：This research was supported by the State Forestry Administration(98 -4 -05);Key Laboratory of Silviculture on the Loess Plateau of State Forestry Administration(K02 -06)

摘　　要：以西黄松成熟胚为外植体诱导不定芽,在GD+9.85～19.70μmol.L-16-BA+0.0～14.42μmol.L-1NAA上不定芽诱导率最高达65.8%,平均增殖率为7,最大增殖率达10;不定芽形成有2种途径,即子叶直接形成不定芽和子叶组织再分化形成不定芽;NAA不利于外植体不定芽的诱导;不定芽的生长和扩繁采用不加生长调节剂的1/2GD和1/2SH培养基;培养基中加入适量的活性炭有利于不定芽和根的生长。不定嫩梢在1/2GD和1/2SH附加不同浓度NAA和GA3的培养基上进行生根诱导,试验结果表明:NAA对不定根的形成起主要作用,在1/2GD+28.84μmol.L-1NAA+4.17μmol.L-1GA3培养基中不定梢的生根率为16.7%。在离体培养条件下,以西黄松成熟胚为外植体获得了再生植株。Adventitious buds were induced from the excised mature zygotic embryos of Ponderosa Pine （ Pinus ponderosa）. The highest induction rate （65.8%） of adventitious buds was obtained with 10-fold multiplication at the highest and 7-fold multiplication at an average when mature zygotic embryos were cultured on GD ＋ 9.85 - 19.70 μmol·L^-1 6-BA ＋ 0.0 - 14.42 μmol·L^-1 NAA. The adventitious buds were initiated or formed directly from the cotyledons. Additional NAA inhibited the formation of adventitious buds. Buds proliferation and elongation were achieved on 1/2GD and 1/2SH without growth regulators. A proper amount of activated charcoal promoted adventitious buds elongation and root growth. The adventitious shoots of Ponderosa Pine were used to induce adventitious roots on 1/2GD and 1/2SH media supplemented with different concentrations of NAA and GA3 . The results showed that NAA played a determinative role in initiating the adventitious roots. Sixteen point seven percent （ 16.7% ） of adventitious shoots was rooted on 1/2GD with 28.84 μmol·L^-1 NAA ＋ 4.17 μmol·L^-1 GA3 . In the present paper, the experimental results showed that roots were successfully induced from cultured mature zygotic embryos of Ponderosa Pine.

关 键 词：西黄松 成熟胚 离体培养 植株再生 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]