家蚕灰僵病病原的鉴定  

Pathogen Identification of Grey Muscardine of the Silkworm,Bombyx mori

作　　者：黄勃[1] 王四宝[1] 金伟[2] 樊美珍[1] 李增智[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036 [2]浙江大学特种经济动物科学系,杭州310029

出　　处：《蚕业科学》2008年第2期257-261,共5页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(编号30770008);新世纪优秀人才支持计划(编号05-0560);安徽省优秀青年科技基金(编号04041043);安徽省级自然科学研究重点项目(编号TD200708)

摘　　要：家蚕灰僵病(grey muscardine)是一类不常见的真菌病,其病原一直未能确定。从感染灰僵病的家蚕体中分离获得一株棒束孢(Isaria),菌株编号为RCEF197,测定了该菌株的ITS1-5.8S-ITS2 rDNA区域序列,研究了该菌株的菌落和显微形态特征。与一些近似的棒束孢和拟青霉(Paecilomyces)的ITS序列进行比较,该菌株和爪哇棒束孢菌株CBS134.22的相似性为99.8%,在构建的棒束孢属部分种的系统发育树中,该菌株与爪哇棒束孢聚为一类,再结合该菌株具有的淡灰紫色菌落和长椭圆形分生孢子大小等形态学特征,将家蚕灰僵病的病原确定为爪哇棒束孢Isaria javanica。The grey muscardine of the silkworm is a kind of uncommon mycosis, and its pathogen hasn＇t been identified. A Isaria strain, RCEF197, was isolated from a silkworm cadaver died of grey muscardine. The ITS1- 5.8S-ITS2 rDNA region from RCEF197 was sequenced, and the colonial and microscopic characters of RCEF197 were investigated. Comparison analysis of the ITS with that of some close species of Isaria and Paecilomyces, it has 99.8% identity to Isaria javanica strain CBS134.22. Phylogenetic tree based on ITS region sequence data from Isaria spp showed that RCEF197 was clustered together with Isaria javanica. Further- more, combined with the morphological characters, the pathogen of grey muscardine of the silkworm should be identified as Isaria javanica.

关 键 词：家蚕 灰僵病 真菌病 爪哇棒柬孢 转录间区 

分 类 号：S884[农业科学—特种经济动物饲养]