银杏黄酮对活性氧自由基和过氧化氢清除作用的研究  

Free radicals and hydrogen peroxide scavenging effect of flavonoid extract from Ginkgo biloba leaves

作　　者：吕平[1] 林明涛[2] 杨柳枝[1] 

机构地区：[1]天津职业大学生物与环境工程学院,天津300072 [2]中国林业科学研究院林产化学工业研究所,江苏南京210042

出　　处：《福建林业科技》2008年第2期116-118,125,共4页Journal of Fujian Forestry Science and Technology

基　　金：天津市高等学校科技发展基金(2005C003)

摘　　要：研究了银杏叶中黄酮类提取物对超氧阴离子自由基、羟自由基清除率和过氧化氢的清除作用。为了进一步分析黄酮类在细胞内的清除作用,在反应体系中加入了大肠杆菌提取物以部分模拟细胞环境。结果表明黄酮类提取物对2类自由基和过氧化氢有明显的清除作用。同时发现在清除过程中,黄酮类与内酯类的相互作用比较复杂。但可以肯定的是大肠杆菌提取物的生物大分子对自由基和过氧化氢的清除没有影响或影响较小。Free radicals and hydrogen peroxide scavenging effect of flavonoid extract from Ginkgo biloba leaves against O2^-, ·OH, hydrogen peroxide was investigated. In contrast, extract from Escherichia coli was added into producing systems of free radicals and hydrogen peroxide in order to show scavenging effect of flavonold extract from G. biloba in cells. It indicated that flavonoid extract from G.biloba leaves showed strong scavenging effect against O2^-, ·OH and hydrogen peroxide. It suggested that mutual function between flavonoid extract and ginkgolide extract from G. biloba leaves was very complex in scavenging process on free radicals and hydrogen peroxide. It also proved that scavenging effect of flavonoid and ginkgolide extract from G. bi/oba leaves against O2^-, ·OH and hydrogen peroxide were not changed by the extract from E. coll.

关 键 词：银杏叶 黄酮类化合物 内酯类化合物 氧自由基 

分 类 号：S792.950.1[农业科学—林木遗传育种] TQ351.01[农业科学—林学]