大肠埃希菌中OXA型超广谱β-内酰胺酶基因分布  

Genotype Distribution of OXA-type Extended-spectrum β-Lactamases in Producing ESBLs Escherichia Coli in Zhengzhou

作　　者：周明莉[1] 阎志勇[2] 郭小兵[3] 

机构地区：[1]郑州铁路局中心卫生防疫站,河南洛阳471002 [2]郑州大学基础医学院,河南郑州450052 [3]郑州大学第一附属医院,河南郑州450052

出　　处：《河南职工医学院学报》2008年第3期217-218,共2页Journal of Henan Medical College For Staff and Workers

基　　金：河南省医学科技攻关项目(编号:200703071)

摘　　要：目的了解郑州地区OXA型ESBLs在大肠埃希菌中基因分布状况。方法收集郑州大学第一附属医院和河南省人民医院2006年1月至2007年10月临床分离产ESBLs大肠埃希菌108株,采用聚合酶链反应(PCR)扩增0XA型ESBLs基因全长序列,采用DNA脱氧核酸测序确定基因型别。结果108株产ESBLs菌株中8株携带OXA型ESBLs基因,测序结果显示8株均为OXA-1型。结论郑州地区大肠埃希菌OXA型ESBLs以OXA-1型为主。Objective To study the genotype distribution of OXA-type ESBLs in producing ESBLs Escherichia coli isolated from two hospitals in Zhengzhou. Methods 108 strains of E. coli were collected from the First Affiliated Hospital of Zhengzhou University and the People＇ s Hospital of Henan Province, from Jan 2006 to Oct 2007. The OXA-type ESBLs genes were amplified by polymerase chain reaction （PCR） ,then to sequence. Results 8 out of 108 ESBLs-producing E. coli isolates were classified with OXA-type-ESBLs genes, they were identified with OXA- 1. Conclusions The population of OXA-1 type ESBLs-producing E. coli exists in Zhengzhou.

关 键 词：OXA ESBLS PCR 基因型 

分 类 号：R378.21[医药卫生—病原生物学]