趋化因子受体CCR4、CCR5在系统性红斑狼疮患者外周血CD4^+T淋巴细胞上的表达及其意义  

Expression of chemokine recepter CCR4 and CCR5 on peripheral blood CD4^+ T cells in systemic lupus erythematosus patients

作　　者：程慧玲[1] 刘建伟[2] 马万山[1] 迟伟玲[1] 

机构地区：[1]山东省千佛山医院检验科,济南250014 [2]山东大学齐鲁医院风湿病科

出　　处：《中华临床医师杂志（电子版）》2008年第6期8-12,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：山东省卫生厅资助项目(2005HZ077)

摘　　要：目的探讨系统性红斑狼疮(SLE)患者趋化因子受体CCR4和CCR5在外周血淋巴细胞表面的表达及其意义。方法流式细胞仪计数法对113例SLE患者和50例健康体检者外周血淋巴细胞表面CCR4和CCR5的表达情况进行检测,分析及评价SLE患者外周血淋巴细胞中CCR4+和CCR5+T淋巴细胞的百分数。结果非狼疮性肾炎(nLN)SLE组外周血CCR4+CD4+T%明显高于健康对照组(P<0.01);狼疮性肾炎SLE组外周血CCR4+CD4+T%明显高于健康对照组(P<0.001);LNSLE组外周血CCR4+CD4+T%明显高于nLNSLE组(P<0.01);nLNSLE组外周血CCR5+CD4+T%高于健康对照组(P<0.05);LNSLE组外周血CCR5+CD4+T%明显高于健康对照组(P<0.001);LNSLE组外周血CCR5+CD4+T%明显高于nLNSLE组(P<0.01)。SLE活动组血清CCR4+CD4+T%与CCR5+CD4+T%较非活动组和对照组明显升高(P<0.01);活动性狼疮性肾炎(LN)与活动性无肾损伤组及对照组比较,其差异具有显著统计学意义(P<0.01)。nLNSLE组外周血CCR4+CD4+T%与CCR5+CD4+T%有相关性(r=0.619,P<0.05);LNSLE组外周血CCR4+CD4+T%与CCR5+CD4+T%有明显相关性(r=0.68,P<0.01);血清CCR4+CD4+T%水平随着SLE疾病活动水平明显升高,与总的系统性红斑狼疮疾病活动性指数(SLEDAI)评分密切相关(r=0.6382,P<0.001);与SLEDAI肾评分亦密切相关(r=0.6980,P<0.001);而CCR5+CD4+T%与疾病活动度不相关(r=0.16,P>0.05)。结论以上结果表明CCR4和CCR5在T细胞趋化至病变部位的过程中可能发挥重要作用,CCR4+CD4+T%与CCR5+CD4+T%可能在肾损伤中起着十分重要的作用,血清CCR4+CD4+T%、CCR5+CD4+T%与SLE疾病活动密切相关,可作为SLE疾病活动,尤其是监测狼疮性肾损伤的重要指标。Objective To explore the expression and significance of chemokine CCR4 and CCR5 on peripheral blood （PB） T cells in systemic lupus erythematosus. Methods Using flow cytometry, we examined the expression of chemokine recepter CCR4 and CCR5 on PB T cells in SLE patients and normal control （NC）, analyzing and assessing the percentage of CCR4 ＋ and CCR5 ＋ CD4 ＋ T cells on PB in SLE patients and NC. Results The percentage of CCR4 ＋ CD4 ＋ T cells on PB was higher significantly in patients with no-LN SLE compared with that of NC （ P 〈 0. 01 ）. The percentage of CCR4 ＋ CD4 ＋ T cells on PB was significantly higher in patients with LN SLE than that of NC（ P 〈 0. 001 ）. The percentage of CCR4 ＋ CD4 ＋ T cells on PB was significantly higher in patients with LN SLE compared with that of no-LN SLE （P 〈0. 01 ）.The percentage of CCR5 ＋ CD4＋ T cells on PB was significantly higher in patients with no-LN SLE compared with that of NC（P 〈 0. 05）. The percentage of CCR5 ＋ CD4 ＋ T cells on PB was significantly higher in patients with LN SLE than that of NC （ P 〈 0. 01 ）. The percentage of CCR5 ＋ CD4 ＋ T cells on PB was significantly higher in patients with LN SLE compared with that of no-LN SLE（ P 〈 0. 01 ）. The percentage of CCR4 ＋ and CCR5 ＋ CD4 ＋T cells on serum in active groups were obviously higher than those in inactive groups and control groups（P 〈 0. 01 ）. Compared with the active no-renal injury groups and control groups,the active LN SLE groups were significantly higher（P 〈 0. 01 ）. The percentage of CCR4 ＋ CD4 ＋ T ceils on PB in on-LN SLE patients have obviously correlation that of CCR5 ＋ CD4 ＋ T cells （ r = 0. 619, P 〈 0. 05 ）. The percentage of CCR4 ＋ CD4 ＋ T ceils on PB in LN SLE patients have correlation with that of CCR5 ＋ CD4 ＋ T cells （ r = 0.680,P 〈 0.01）. The serum CCR4＋ CD4＋ T% levels went up obviously along with the SLE disease activity,the levels of CCR4 ＋ CD4 ＋ T% correlated closely with

关 键 词：红斑狼疮 系统性 受体 CCR4 受体 CCR5 T淋巴细胞 

分 类 号：R593.241[医药卫生—内科学]